2ND-DERIVATIVE SPECTROSCOPY OF PROTEINS - METHOD FOR QUANTITATIVE-DETERMINATION OF AROMATIC AMINO-ACIDS IN PROTEINS
2ND-DERIVATIVE SPECTROSCOPY OF PROTEINS - METHOD FOR QUANTITATIVE-DETERMINATION OF AROMATIC AMINO-ACIDS IN PROTEINS
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DOI：
10.1111/j.1432-1033.1978.tb12622.x
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发表时间：
1978-01-01
期刊：
影响因子：
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通讯作者：
SERVILLO, L
中科院分区：
文献类型：
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作者：
BALESTRIERI, C;COLONNA, G;SERVILLO, L
二阶导数光谱用于解析近紫外区域的复杂蛋白质光谱，并评估了3种芳香族发色团的贡献。建立了一种直接定量测定蛋白质中苯丙氨酸和色氨酸的方法。苯丙氨酸的测定在250和265 nm之间的光谱区域进行，其中没有来自其他芳香族发色团的显著贡献。在290-295 nm区域进行色氨酸测定，并针对酪氨酸的存在对实验值进行了校正。对10种高度纯化的蛋白质[牛血清白蛋白，α- chymotrysinoqen、人Hb、抹香鲸肌红蛋白、卵溶菌酶、牛肝过氧化氢酶、马心细胞色素c、木瓜蛋白酶、牛肉高铁肌红蛋白和脱辅基肌红蛋白]与序列分析结果一致。
Second derivative spectroscopy was used to resolve the complex protein spectrum in the near-UV region and the contributions of the 3 aromatic chromophores were evaluated. A method for the direct quantitative determination of phenylalanine and tryptophan in proteins was carried out. Phenylalanine determination was carried out in the spectral region between 250 and 265 nm, where there are no significant contributions from other aromatic chromophores. Tryptophan determination was performed in the 290-295 nm region and the experimental values have been corrected for the presence of tyrosine. The results obtained on 10 highly purified proteins [bovine serum albumin, .alpha.-chymotrysinoqen, human Hb, sperm whale myoglobin, egg lysozyme, bovine liver catalase, horse heart cytochrome c, papain, beef ferrimyoglobin and apomyoglobin] were in good agreement with those obtained from sequence analysis.