The maternal nucleolus is essential for early embryonic development in mammals
The maternal nucleolus is essential for early embryonic development in mammals
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DOI：
10.1126/science.1151276
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发表时间：
2008-02-01
期刊：
影响因子：
56.9
通讯作者：
Fulka, Josef, Jr.
中科院分区：
文献类型：
--
作者：
Ogushi, Sugako;Palmieri, Chiara;Fulka, Josef, Jr.
在受精过程中，父方和母方对合子的贡献是不相等的。卵母细胞和精子具有形成发育能力胚胎所必需的细胞结构和分子的互补库。我们表明，核仁是专为母亲的起源。母体核仁不是卵母细胞成熟所必需的;然而，它是受精或孤雌激活后形成原核核仁所必需的，并且是进一步胚胎发育所必需的。此外，通过体细胞核移植产生的胚胎中的核仁来源于卵母细胞，表明母体核仁支持成功的胚胎发育。
With fertilization, the paternal and maternal contributions to the zygote are not equal. The oocyte and spermatozoon are equipped with complementary arsenals of cellular structures and molecules necessary for the creation of a developmentally competent embryo. We show that the nucleolus is exclusively of maternal origin. The maternal nucleolus is not necessary for oocyte maturation; however, it is necessary for the formation of pronuclear nucleoli after fertilization or parthenogenetic activation and is essential for further embryonic development. In addition, the nucleolus in the embryo produced by somatic cell nuclear transfer originates from the oocyte, demonstrating that the maternal nucleolus supports successful embryonic development.