Isolation and characterization of thermotolerant bacterium utilizing ammonium and nitrate ions under aerobic conditions
Isolation and characterization of thermotolerant bacterium utilizing ammonium and nitrate ions under aerobic conditions
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DOI：
10.1007/s10529-006-9255-8
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发表时间：
2007-01
影响因子：
2.7
通讯作者：
S. Takenaka;Qi Zhou;Ampin Kuntiya;Phisit Seesuriyachan;S. Murakami;K. Aoki
中科院分区：
文献类型：
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作者：
S. Takenaka;Qi Zhou;Ampin Kuntiya;Phisit Seesuriyachan;S. Murakami;K. Aoki
地衣芽孢杆菌（Bacilluslicheniformis）是一株耐热细菌，在30和50°C有氧条件下，能完全利用0.1%（w/v）的NH_4NO_3。在NH 4 NO3培养基中添加0.5 mM Fe 2+显著促进了NH 4+和NO3−的利用。在50°C下，最初提供的总氮的24%被吸收到细胞中，20%保留在培养物上清液中。残留的氮（56%）可能被排放到大气中。细胞提取物含有参与脱氮的酶。GC-MS证明NH 415 NO3已转化为15 N2 O。这些结果表明该菌株在好氧条件下具有反硝化能力。
A thermotolerant bacterium, identified asBacillus licheniformis, completely utilized 0.1% (w/v) NH4NO3at 30 and 50°C under aerobic condition. The addition of 0.5 mM Fe2+to the NH4NO3medium markedly promoted the utilization of NH4+and NO3−. At 50°C, of total nitrogen originally provided, 24% was taken up into the cells and 20% remained in the culture supernatant. Residual nitrogen (56%) was probably removed into the atmosphere. The cell extracts contained enzymes involved in denitrification. GC-MS demonstrated that NH415NO3had been converted to15N2O. These results indicate that the strain has denitrification ability under aerobic condition.