Mechanical properties and biocompatibility of titanium with a high oxygen concentration for dental implants
Mechanical properties and biocompatibility of titanium with a high oxygen concentration for dental implants
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牙种植体用高氧浓度钛的机械性能和生物相容性
DOI：
10.1016/j.msec.2020.111306
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发表时间：
2020-12-01
影响因子：
7.9
通讯作者：
Shen, Jianghua
中科院分区：
文献类型：
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作者：
Luo, Huiwen;Wu, Yulu;Shen, Jianghua
为了提高口腔种植体用工业纯钛（CP-Ti）的强度，采用粉末冶金法制备了高氧含量纯钛（HOC-Ti）。然后对其组成和力学性能进行了表征。通过喷砂和酸蚀（SLA）对HOC-Ti和CP-Ti试样进行表面处理，研究了试样的表面形貌、润湿性和粗糙度。在体外试验中，HOC-Ti的蛋白质吸附能力的评价，小鼠前成骨细胞接种到标本上，以评估其生物相容性，与CP-Ti相比。HOC-Ti的氧浓度增加到0.62wt%，这高于CP-Ti的0.26wt%，而它们的组成和微观结构非常相似。HOC-Ti的拉伸和压缩屈服强度（800 MPa）与CP-Ti的拉伸和压缩屈服强度（530 MPa）相比显著提高。表面处理后，在HOC-Ti的整个表面上观察到直径为380 nm的独特微孔结构，其有利于细胞粘附和增殖。HOC-Ti的润湿性明显上级（p < 0.05）。体外实验结果表明，接种于HOC-Ti表面的MC 3 T3-E1细胞具有均匀的显微结构，在第4天和第7天的存活率高于对照组（p < 0.05）。此外，粘附到HOC-Ti表面的细胞的数量和分化活性在第7天显著增加（p < 0.05）。实验结果表明，从力学性能和生物相容性来看，HOC-Ti上级CP-Ti，有望用于口腔种植体。
In order to improve the strength of commercially pure Ti (CP-Ti) for oral implants, the high oxygen content Ti (HOC-Ti) was prepared via powder metallurgy. Its composition and mechanical properties were then characterized. After surface treatment by sandblasting and acid etching (SLA), the surface morphology, wettability and roughness of the HOC-Ti and CP-Ti sample were examined. In an in vitro test that followed an evaluation of the protein adsorption capacity of HOC-Ti, the mouse preosteoblast cells were inoculated onto the specimens to evaluate their biocompatibility, in comparison with those of CP-Ti. The oxygen concentration of the HOC-Ti increased to 0.62 wt%, which is higher than the 0.26 wt% of the CP-Ti, while their compositions and microstructures were very similar. The tensile and compressive yield strength of the HOC-Ti (800 MPa) was improved significantly in comparison to that of the CP-Ti (530 MPa). After surface treatment, a unique structure of micropores with a diameter of 380 nm was observed on the entire surface of the HOC-Ti that facilitates cell adhesion and proliferation. The wettability of the HOC-Ti was obviously superior (p < 0.05). The in vitro study showed that the MC3T3-E1 cells inoculated on the surface of HOC-Ti exhibited a homogeneous microstructure, and the viability was higher than that of the control group on days 4 and 7 (p < 0.05). In addition, the number and differentiation activity of cells that adhered to the surface of the HOC-Ti increased significantly on day 7 (p < 0.05). The experimental results showed that, in view of its mechanical properties and biocompatibility, HOC-Ti is superior to CP-Ti and is promising for oral implant applications.