Comprehensive analysis of the phospholipids and phytosterols in Schisandra chinensis oil by UPLC-Q/TOF- MSE
Comprehensive analysis of the phospholipids and phytosterols in Schisandra chinensis oil by UPLC-Q/TOF- MSE
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UPLC-Q/TOF-MSE 综合分析五味子油中的磷脂和植物甾醇
DOI：
10.1016/j.chemphyslip.2019.03.003
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发表时间：
2019-07-01
影响因子：
3.4
通讯作者：
Wu, Shimin
中科院分区：
文献类型：
--
作者：
Gao, Yuan;Wu, Shimin
采用UPLC-Q/TOF-MSE对五味子挥发油中的磷脂和植物甾醇进行了表征。确定过程基于由MSE检测器生成的部件的特征片段信息。在阴离子和阳离子模式下，分别鉴定出49个和39个磷脂酶。分类包括磷脂酸(PAS)、磷脂酰乙醇胺(PES)、磷脂酰甘油(PGs)、磷脂酰肌醇(PI)、磷脂酰丝氨酸(PSS)和磷脂酰胆碱(PC)。Pis和PCs种类最多，分别占12种和14种。定量分析表明，五味子油中含量最丰富的成分为PES和PCs，分别为122.85+/-3.82和85.61+/-2.12mgg/g。此外，在阳离子模式下初步鉴定出紫菜油中含有13种植物甾醇，其中锥形甾醇C含量最高，为22.02+/-0.98mU g/g，紫菜油中还检测到油菜素、油菜籽甾醇、植物甾醇B和草甾醇。这些结果对黄连油的质量评价和功能评价具有一定的参考价值。
Characterization of phospholipids (PLs) and phytosterols were determined in Schisandra chinensis (S. chinensis) oil by UPLC-Q/TOF-MSE. The determination process was based on feature fragment information of components generated by the MSE detector. A total of 49 and 39 PLs were identified in S. chinensis oil under negative and positive ion mode, respectively. The classes of PLs included phosphatidic acids (PAs), phosphatidylethanolamines (PEs), phosphatidylglycerols (PGs), phosphatidylinositols (PIs), phosphatidylserines (PSs) and phosphatidylcholines (PCs). The most diverse species of PLs detected were PIs and PCs, accounting for 12 and 14, respectively. The analysis of quantification indicated that PEs and PCs were the most abundant constituents in S. chinensis oil, accounting for 122.85 +/- 3.82 and 85.61 +/- 2.12 mu g/g, respectively. Besides, thirteen kinds of phytosterols were tentatively identified in S. chinensis oil under positive ion mode, among which, conicasterol C was the most abundant component, accounting for 22.02 +/- 0.98 mu g/g. Brassicasterol, campesterol, secosterol-B and herbasterol were also determined in S. chinensis oil. These results might be meaningful in the quality assessment and function evaluation of S. chinensis oil.