Arabidopsis Glucan Synthase-Like1 (GSL1) Is Required for Tolerance to Low-Calcium Conditions and Exhibits a Function Comparable to GSL10
Arabidopsis Glucan Synthase-Like1 (GSL1) Is Required for Tolerance to Low-Calcium Conditions and Exhibits a Function Comparable to GSL10
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拟南芥葡聚糖合成酶样 1 (GSL1) 是耐受低钙条件所必需的，并且表现出与 GSL10 相当的功能
DOI：
10.1093/pcp/pcac106
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发表时间：
2022
影响因子：
4.9
通讯作者：
Fujiwara Toru
中科院分区：
文献类型：
--
作者：
Shikanai Yusuke;Takahashi Satomi;Enomoto Yusuke;Yamagami Mutsumi;Yamaguchi Katsushi;Shigenobu Shuji;Kamiya Takehiro;Fujiwara Toru
表现出钙（Ca）缺乏症状的作物构成主要的农业问题。抗病品种的分子育种是解决这一问题的有效途径。然而，首先必须确定相关基因。在这里，我们表明，葡聚糖糖苷酶样（GSL）1基因是必需的低钙耐受拟南芥。GSL 1与GSL 10同源，GSL 10在低钙耐受中起重要作用。在低钙条件下，gsl1突变体表现出生长减少和新叶坏死的发病。这些症状是缺钙作物的典型症状。嫁接实验表明，芽基因型，而不是根基因型，是重要的抑制芽坏死。与野生型植株相比，突变体在低钙条件下胼胝质的异位积累显著减少。由于相应的单突变体表型相似，我们通过测试gsl1 gsl10双突变体对低钙条件的敏感性来研究GSL 1和GSL 10之间的相互作用。双突变体表现出比单突变体更严重的表型，表明GSL 1和GSL 10对低钙耐受性的作用是加性的。由于GSL基因在植物界中高度保守，GSL基因座可用于培育耐低钙作物。
Crops that exhibit symptoms of calcium (Ca) deficiency constitute a major agricultural problem. Molecular breeding of resistant cultivars is a promising method for overcoming this problem. However, the involved genes must first be identified. Here, we show that theglucan synthase-like(GSL)1gene is essential for low-Ca tolerance inArabidopsis thaliana. GSL1is homologous toGSL10, which we previously showed was essential for low-Ca tolerance. Under low-Ca conditions,gsl1mutants exhibit reduced growth and the onset of necrosis in new leaves. These symptoms are typical of Ca-deficient crops. A grafting experiment suggested that the shoot genotype, but not the root genotype, was important for the suppression of shoot necrosis. The ectopic accumulation of callose under low-Ca conditions was significantly reduced ingsl1mutants compared with wild-type plants. Because the corresponding single-mutant phenotypes are similar, we investigated the interaction betweenGSL1andGSL10by testing thegsl1 gsl10double mutant for sensitivity to low-Ca conditions. The double mutant exhibited a more severe phenotype than did the single mutants, indicating that the effects ofGSL1andGSL10on low-Ca tolerance are additive. BecauseGSLgenes are highly conserved within the plant kingdom, theGSLloci may be useful for breeding low-Ca tolerant crops.