Multiplex real-time PCR assays to detect Stenotrophomonas maltophilia carrying sul1, sul2, and sul3 genes
Multiplex real-time PCR assays to detect Stenotrophomonas maltophilia carrying sul1, sul2, and sul3 genes
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多重实时 PCR 检测携带 sul1、sul2 和 sul3 基因的嗜麦芽寡养单胞菌
DOI：
10.1016/j.mimet.2018.12.002
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发表时间：
2019-01-01
影响因子：
2.2
通讯作者：
Rui, Yongyu
中科院分区：
文献类型：
--
作者：
Li, Si;Peng, Yuan;Rui, Yongyu
由耐SXT的嗜麦芽窄食单胞菌引起的医院感染在全球范围内呈上升趋势。本研究首次建立了一种新的基于熔融曲线的多重实时荧光聚合酶链式反应方法，用于同时检测srA和sul1、sul2和sul3基因。检测在罗氏LightCycler(R)480 II系统上进行。对靶基因和非靶基因扩增的结果表明，多重实时荧光定量聚合酶链式反应是特异的，每个靶基因的检测下限为每20亩L反应体积10个拷贝，目的基因的6个对数稀释度(r(2)>；0.99)呈线性关系，批内和批间重复性变异系数的Ct值为
Nosocomial infections caused by Stenotrophomonas maltophilia resistant to SXT are increasingly reported worldwide. In this study, a novel melting-curve based multiplex real-time PCR assay for the simultaneous detection of the ssrA and sul1, sul2 and sul3 genes was first established. The assays were performed on a Roche LightCycler (R) 480 II system. The results for target and non-target amplification showed that the multiplex real-time PCR assays were specific, the limit of detection for each target was 10 copies per 20 mu L reaction volume, the assays were linear over six log dilutions of the target genes (r(2) > 0.99), and the Ct values of the coefficients of variation for intra- and interassay reproducibility were