Growth hormone increases ovarian levels of immunoreactive somatomedin C/insulin-like growth factor I in vivo.
Growth hormone increases ovarian levels of immunoreactive somatomedin C/insulin-like growth factor I in vivo.
复制标题
生长激素可增加体内免疫反应性生长调节素 C/胰岛素样生长因子 I 的卵巢水平。
作者：
J. Davoren;AARON J.W. Hsdeh
GH可能通过增加GH依赖的生长激素C/胰岛素样生长因子I （IGF I）的局部水平来影响性腺功能的假设得到了验证。将羊GH（200微克）注射到未成熟、去垂体、雌激素处理的雌性大鼠体内；动物在8或12小时后被处死。肾和卵巢均质液酸提取，用Sephadex G-50进行色谱分析。注射生长激素8小时后，经酸层析处理的卵巢或肾脏提取物中免疫反应性IGF I （IR-IGF I）水平升高3.6 ~ 6.4倍。生长激素治疗12小时后，IR-IGF I水平仍然升高，但低于8小时后的水平。在这两种情况下，IR-IGF I水平都不能由血清污染来解释。在肾脏和卵巢中，IR-IGF I洗脱后的表观摩尔重量接近合成人IGF I。因此，生长激素在体内可直接提高卵巢和肾组织IR-IGF I水平。结合之前在体外对大鼠颗粒细胞的直接促性腺激素增强作用的观察，我们的研究结果支持了生长激素可能通过诱导IGF I的局部产生或积累来影响卵巢分化的假设，为新的卵巢内旁分泌控制机制提供了证据。
The hypothesis that GH may affect gonadal function by increasing local levels of the GH-dependent somatomedin C/insulin-like growth factor I (IGF I) was tested. Ovine GH (200 micrograms) was injected into immature, hypophysectomized, estrogen-treated female rats; animals were sacrificed 8 or 12 h later. Renal and ovarian homogenates were acid extracted and chromatographed over Sephadex G-50. Eight h after GH injection, 3.6 to 6.4-fold increases in immunoreactive IGF I (IR-IGF I) levels were observed in either ovarian or renal extracts subjected to acid chromatography. Twelve h after GH treatment, IR-IGF I levels remained elevated, but were lower than after 8 h. In neither case could IR-IGF I levels be accounted for by serum contamination. IR-IGF I eluted with an apparent mol wt near that of synthetic human IGF I in both kidney and ovary. Thus, GH can directly increase ovarian and renal tissue IR-IGF I levels in vivo. Taken with previous observations showing a direct gonadotropin-enhancing effect of IGF I on rat granulosa cells in vitro, our results support the hypothesis that GH may affect ovarian differentiation by inducing the local production or accumulation of IGF I, providing evidence for a novel intraovarian paracrine control mechanism.
培养的人成纤维细胞产生免疫反应性生长调节素的激素控制。
DOI：
10.1172/jci110001
发表时间：
1981
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Clemmons,DR;Underwood,LE;VanWyk,JJ
通讯作者：
VanWyk,JJ
将胰岛素样生长因子和结合蛋白活性释放到培养的人成纤维细胞的无血清培养基中。
影响因子：
4.8
作者：
Adams,SO;Kapadia,M;Mills,B;Daughaday,WH
通讯作者：
Daughaday,WH