Re-Formation of the Preprophase Band After Cold-Induced Depolymerization of Microtubules in Adiantum Protonemata
Re-Formation of the Preprophase Band After Cold-Induced Depolymerization of Microtubules in Adiantum Protonemata
复制标题
铁线蕨微管冷诱导解聚后前期带的重建
DOI：
10.1093/oxfordjournals.pcp.a078191
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发表时间：
1991
影响因子：
4.9
通讯作者：
M. Wada
中科院分区：
文献类型：
--
作者：
T. Murata;M. Wada
研究了羊齿蕨（Adiantum capilus - venerisl）原细胞冷诱导解聚后微管（MTs）前前期带（PPB）的重建。当带PPB的原体在0℃下冷冻时，PPB的MTs在5 min内解聚，只留下少量的MTs片段，所有这些片段在冷处理开始后的2 h内被破坏。细胞冷却5 min后，在25℃下再次加热，PPB区域的横向mt数量逐渐增加，PPB在20 min内完成重组。相反，当细胞冷却2 h后重新加热时，最初出现随机定向的mt，然后出现低密度mt的PPB。即使通过离心（2800 ×g， 15分钟）使细胞核和大部分内质从PPB区域移位，PPB也会重新形成。这些结果表明，在PPB被破坏后重新形成的过程中，PPB的mt在皮质细胞质中形成核和组织。
Re-formation of the preprophase band (PPB) of microtubules (MTs) after cold-induced depolymerization of MTs was investigated in protonemal cells of the fern,Adiantum capiilus-venerisL. When protonemata with a PPB were chilled at 0°C, MTs of the PPB depolymerized within 5 min leaving only a few fragments of MTs, and all such fragments were disrupted within 2 h after the onset of cold treatment. When cells were chilled for 5 min and then rewarmed at 25 °C, the transverse MTs of the PPB gradually increased in number in the region of the PPB and re-formation of the PPB was accomplished within 20 min. In contrast, when cells chilled for 2 h were rewarmed, randomly oriented MTs appeared initially and then a PPB with a low density of MTs became apparent. The PPB re-formed even when the nucleus and most of the endoplasm had been displaced from the region of the PPB by centrifugation (2,800 ×g, 15 min). These results suggest that MTs of the PPB nucleate and are organized in the cortical cytoplasm during re-formation of the PPB after disruption.