Improving polyhydroxyalkanoate production by knocking out the genes involved in exopolysaccharide biosynthesis in Haloferax mediterranei
Improving polyhydroxyalkanoate production by knocking out the genes involved in exopolysaccharide biosynthesis in Haloferax mediterranei
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通过敲除 Haloferax mediterranei 胞外多糖生物合成所涉及的基因来提高聚羟基脂肪酸酯的产量
DOI：
10.1007/s00253-012-4415-3
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发表时间：
2012-09
影响因子：
5
通讯作者：
Xiang H
中科院分区：
文献类型：
--
作者：
Zhao D;Cai L;Wu J;Li M;Liu H;Han J;Zhou J;Xiang H
地中海盐法克菌（Haloferax meditaliacei）能利用多种碳源大量合成聚（3-羟基丁酸-co-3-羟基戊酸）（PHBV），并产生胞外多糖（EPS）。在这项研究中，我们确定了一个基因簇参与EPS生物合成的H。医生。敲除该簇中编码推定的UDP-N-乙酰葡糖胺6-脱氢酶（HFX_2145）、糖基转移酶（HFX_2146和HFX_2147）和多糖转运蛋白（HFX_2148）的基因消除了EPS合成。突变株EPS生物合成的缺陷显著降低了发酵液的粘度，从而增加了溶解氧含量，降低了起泡性。与野生型（WT）菌株相比，EPS突变菌株的PHBV生产显著增强（约20%），而在相同培养条件下细胞生长速率保持相似。这些结果表明，用于合成EPS的碳源已转移到PHBV生产中。因此，一种新的工程H.该菌株为PHBV的工业化生产奠定了基础。
Haloferax mediterranei is capable of producing large amounts of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from many kinds of carbon sources, with exopolysaccharide (EPS) as a by-product. In this study, we identified a gene cluster involved in EPS biosynthesis in H. mediterranei. Knocking out the genes in this cluster encoding the putative UDP-N-acetylglucosamine 6-dehydrogenase (HFX_2145), glycosyltransferases (HFX_2146 and HFX_2147) and polysaccharide transporter (HFX_2148) eliminated EPS synthesis. The deficiency in EPS biosynthesis in the mutant strain remarkably decreased the viscosity of culture broth, and hence increased the dissolved oxygen content and decreased the foaming propensity. Compared with the wild-type (WT) strain, the PHBV production of the EPS-mutant strain was significantly enhanced (approximately 20%), whereas the cell growth rate remained similar under the same culture conditions. These results indicated that the carbon sources used for synthesizing EPS were shifted to PHBV production. Thus, a novel engineered H. mediterranei strain was developed, which would be favorable for future industrial production of PHBV.
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影响因子：
4.6
作者：
Boan, IF;Garcia-Quesada, JC;Marcilla, A
通讯作者：
Marcilla, A
影响因子：
4.4
作者：
Booth, Sean C.;Workentine, Matthew L.;Weljie, Aalim M.
通讯作者：
Weljie, Aalim M.
影响因子：
4.4
作者：
Cai, Shuangfeng;Cai, Lei;Xiang, Hua
通讯作者：
Xiang, Hua
影响因子：
4.1
作者：
Mishra, P;Srivastava, P;Kundu, S
通讯作者：
Kundu, S
代谢多功能嗜盐古菌 Haloferax mediterranei（一种聚（3-羟基丁酸酯-co-3-羟基戊酸酯）生产者）的完整基因组序列
影响因子：
3.2
作者：
Han, Jing;Zhang, Fan;Xiang, Hua
通讯作者：
Xiang, Hua