DEDIFFERENTIATED CHONDROCYTES REEXPRESS THE DIFFERENTIATED COLLAGEN PHENOTYPE WHEN CULTURED IN AGAROSE GELS
DEDIFFERENTIATED CHONDROCYTES REEXPRESS THE DIFFERENTIATED COLLAGEN PHENOTYPE WHEN CULTURED IN AGAROSE GELS
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DOI：
10.1016/0092-8674(82)90027-7
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发表时间：
1982-01-01
期刊：
影响因子：
64.5
通讯作者：
SHAFFER, JD
中科院分区：
文献类型：
--
作者：
BENYA, PD;SHAFFER, JD
兔关节软骨细胞的分化表型主要由II型胶原蛋白和软骨特异性蛋白聚糖组成。在串行单层培养物中，这种表型被丢失并取代了一个复杂的胶原蛋白表型，该表型主要由I型胶原蛋白和低水平的蛋白聚糖合成。这种去分化的chrondrocytes在液体凝胶中悬浮培养期间的分化表型在0.5％低的TM琼脂糖中。大约80％的细胞从锚固依赖性培养的扁平形态转变为锚固非依赖性培养物的球形形态，然后沉积特征性蛋白聚糖基质域。蛋白聚糖和胶原蛋白合成的速率恢复为原发软骨细胞的速率。十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳的完整胶原蛋白链和二维氰基溴化物肽映射显示出完全回到分化的胶原蛋白表型。这些结果强调了细胞形状在软骨细胞表型的调节中的主要作用，并证明了用于研究基因表达的可逆系统。
The differentiated phenotype of rabbit articular chondrocytes consists primarily of type II collagen and cartilage-specific proteoglycan. During serial monolayer culture this phenotype is lost and replaced by a complex collagen phenotype consisting predominately of type I collagen and a low level of proteoglycan synthesis. Such dedifferentiated chrondrocytes reexpress the differentiated phenotype during suspension culture in firm gels of 0.5% low Tm agarose. Approximately 80% of the cells survive this transition from the flattened morphology of anchorage-dependent culture to the spherical morphology of anchorage-independent culture and then deposit characteristic proteoglycan matrix domains. The rates of proteoglycan and collagen synthesis return to those of primary chondrocytes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of intact collagen chains and 2-dimensional cyanogen bromide peptide mapping demonstrated a complete return to the differentiated collagen phenotype. These results emphasize the primary role of cell shape in the modulation of the chondrocyte phenotype and demonstrate a reversible system for the study of gene expression.