Study of Differentiation Level by Three-dimensional Culture of Mouse ES Cells on Titanium Alloy Powder
Study of Differentiation Level by Three-dimensional Culture of Mouse ES Cells on Titanium Alloy Powder
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钛合金粉末三维培养小鼠ES细胞分化水平研究
DOI：
10.11223/jarde.15.159
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Nishikawa T.
中科院分区：
文献类型：
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作者：
Imai K;Shirai T;Akiyama M;Zenyu M;Yoshida T;Okamura T;Shida M;Shimizu H;Morita S;Tominaga K;Masuno K;Matsumoto H;Nishikawa T.
纯钛表面作为牙科种植体植入时与骨结合牢固，与种植体周围软组织具有高度生物相容性。但Ti-6Al-4V合金的力学强度上级纯钛。钛合金产品约占日本所有植入物产品的40%。在Ti-6Al-4V合金的组成元素中，钒的毒性最大。Ti-6Al-4V合金用于各种钛合金植入物产品。在本研究中，在胶原蛋白上的三维ES细胞培养中，使用抛光Ti-6Al-4V合金检查细胞分化和增殖水平，证明Ti-6Al-4V合金无发育毒性。而经氢氟酸溶液腐蚀的Ti-6Al-4V合金粉末对ES细胞的分化和增殖有影响。
Pure titanium surface is strongly bound to the bone when embedded as a dental implant, with high biocompatibility to the soft tissue around the implant. However, the mechanical strength of Ti-6Al-4V alloy is superior to that of pure titanium. Titanium alloy products account for about 40% of all implant products in Japan. Of the composition elements of Ti-6Al-4V alloy, vanadium has a higher toxicity than the others. Ti-6Al-4V alloys are employed in various titanium alloy implant products. In the present study, cell differentiation and proliferation levels were examined with a polished Ti-6Al-4V alloy in three-dimensional ES cell culture on collagen, demonstrating no developmental toxicity of Ti-6Al-4V alloy. However, Ti-6Al-4V alloy powder corroded with a hydrofluoric acid solution had effects on the differentiation and proliferation of ES cells.