GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR IS A GROWTH-FACTOR FOR PROMASTIGOTES OF LEISHMANIA-MEXICANA-AMAZONENSIS
GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR IS A GROWTH-FACTOR FOR PROMASTIGOTES OF LEISHMANIA-MEXICANA-AMAZONENSIS
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DOI：
10.1111/j.1550-7408.1990.tb01157.x
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发表时间：
1990-09-01
期刊：
影响因子：
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通讯作者：
BARCINSKI, MA
中科院分区：
文献类型：
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作者：
CHARLAB, R;BLAINEAU, C;BARCINSKI, MA
在本文中，我们表明，小鼠肺条件培养基（LCM）显示，除了其已经描述的骨髓细胞集落刺激活性，一个强大的生长刺激活性的墨西哥利什曼原虫亚马逊前鞭毛体。LCM与鼠粒细胞-巨噬细胞集落刺激因子（GM-CSF）特异性抗体的免疫沉淀消除了这两种活性，表明利什曼原虫生长促进活性是由于LCM上存在GM-CSF。此外，添加到培养基或免疫沉淀的LCM中的重组GM-CSF（rGM-CSF）能够分别诱导或部分恢复LCM的促生长活性。寄生虫的体外连续传代诱导对生长因子的敏感性逐渐丧失。最近从病变处收集的寄生虫形式比已经适应在培养物中生长的形式对生长因子的反应明显更大。由于它已被证明，几种不同的微生物显示受体的脊椎动物样激素和GM-CSF是能够增强皮肤利什曼病病变，我们的研究结果使我们能够提出的假设，宿主衍生的激素和病原微生物之间的直接相互作用可以是重要的，在定义感染的命运。GM-CSF是由积极参与利什曼原虫感染的细胞（T淋巴细胞和巨噬细胞）产生的这一事实加强了我们的假设。
In this paper we show that murine lung conditioned medium (LCM) displays, in addition to its already described colony-stimulating activity on bone marrow cells, a potent growth-stimulating activity on promastigotes of Leishmania mexicana amazonensis. Immunoprecipitation of LCM with an antibody specific for murine granulocyte-macrophage colony stimulating factor (GM-CSF) abrogates both activities, indicating that the leishmanial growth-promoting activity is due to the presence of GM-CSF on LCM. Furthermore, recombinant GM-CSF (rGM-CSF) added to the culture medium or to the immunoprecipitated LCM is able to respectively induce or to partially recover the growth-promoting activity of the LCM. Sequential in vitro passages of the parasite induces a progressive loss of sensitivity to the growth-factor. Parasite forms recently collected from lesions are significantly more responsive to the growth-factor than forms already adapted to grow in culture. Since it has been shown that several different microorganisms display receptors for vertebrate-like hormones and that GM-CSF is able to enhance a cutaneous leishmanial lesion, our results permit us to raise the hypothesis that a direct interaction between a host-derived hormone and a pathogenic microorganism can be of importance in defining the fate of an infection. The fact that GM-CSF is produced by cells that actively participate in a leishmanial infection (T-lymphocytes and macrophages) reinforces our hypothesis.