Similarity of junctions between plasma membranes and endoplasmic reticulum in muscle and neurons.
Similarity of junctions between plasma membranes and endoplasmic reticulum in muscle and neurons.
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DOI：
10.1083/jcb.70.2.338
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发表时间：
1976-08
影响因子：
7.8
通讯作者：
Reese, T S
中科院分区：
文献类型：
--
作者：
Henkart, M;Landis, D M;Reese, T S
采用冷冻断裂技术研究了文昌鱼肌和小鼠小脑神经元内质网膜与下贮池连接处的膜结构。在文昌鱼肌中，肌浆网的表面下池与肌节I带水平的表面膜形成连接。在覆盖这些连接处的肌膜的原生质小叶上是大颗粒的聚集体。在小脑篮细胞、星状细胞和普尔基尼细胞膜的原生质小叶上有类似的大颗粒聚集体。在这两种组织中，相应的外膜半部分具有明显与大颗粒聚集体互补的凹坑阵列。通过连接处的交叉断裂表明，神经元和肌肉膜上的颗粒聚集体一致位于紧贴质膜的细胞内池上方。因此，在哺乳动物神经元和文昌鱼肌肉的地下池中发现了类似的质膜特化。这种相似性支持了一种假设，即神经元的表面下蓄水池，就像肌肉中的蓄水池一样，将一些细胞内活动与质膜的电活动耦合在一起。
The structure of membranes at junctions between the plasma membrane and underlying cisterns of endoplasmic reticulum in amphioxus muscle and mouse cerebellar neurons was studied using the freeze-fracture technique. In amphioxus muscle, subsurface cisterns of sarcoplasmic reticulum form junctions with the surface membrane at the level of the sarcomere I bands. On the protoplasmic leaflet of the sarcolemma overlying these junctions were aggregates of large particles. On the protoplasmic leaflet of the membranes of cerebellar basket, stellate and Purkinie cells there were similar aggregates of large particles. In both tissues, the corresponding external membrane halves had arrays of pits apparently complementary to the aggregates of large particles. Cross fractures through junctions showed that the particle aggregates in neuronal and muscle membranes were consistently located over intracellular cisterns closely applied to the plasma membrane. Thus, a similar plasma membrane specialization is found at subsurface cisterns in mammalian neurons and amphioxus muscle. This similarity supports the hypothesis that subsurface cisterns in neurons, like those in muscle, couple some intracellular activity to the electrical activity of the plasma membrane.