Alteromonas tagae sp nov and Alteromonas simiduii sp nov., mercury-resistant bacteria isolated from a Taiwanese estuary
Alteromonas tagae sp nov and Alteromonas simiduii sp nov., mercury-resistant bacteria isolated from a Taiwanese estuary
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DOI：
10.1099/ijs.0.64762-0
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发表时间：
2007-06-01
影响因子：
2.8
通讯作者：
Wagner-Doebler, Irene
中科院分区：
文献类型：
--
作者：
Chiu, Hsiu-Hui;Shieh, Wung Yang;Wagner-Doebler, Irene
从台湾台南市二仁溪河口的水样中分离到两株耐汞的异养好氧海洋细菌，命名为AT 1 T和AS 1（T）。细胞为革兰氏阴性杆菌，通过单个极鞭毛运动。产生芽和假体鞘。这两个分离物需要NaCl生长，并且在约30 ° C、2- 4%NaCl和pH 7-8下生长最佳。它们以需氧方式生长，不能通过发酵葡萄糖或其他碳水化合物进行厌氧生长。它们在含有HgCl 2的液体培养基中生长并表达Hg 2+还原活性。菌株AS 1（T）将硝酸盐还原为亚硝酸盐。主要的类异戊二烯醌是Q（8）（91.3-99.9%）。菌株AT 1（T）的极性脂质由磷脂酰乙醇胺（46.6%）、磷脂酰甘油（28.9%）和硫脂（24.5%）组成，而菌株AS 1（T）的极性脂质由磷脂酰乙醇胺（48.2%）和磷脂酰甘油（51.8%）组成。这两个分离物含有C-16：（1）ω 7 c和/或异-C-15：0 2-OH（22.4-33.7%）、C-16：0（19.0-22.7%）和C-18：1 ω 7 c（11.3-11.7%）作为主要脂肪酸。菌株AT 1（T）和AS 1（T）的DNA G + C含量分别为43.1和45.3 mol%。基于16 S rRNA基因序列的系统发育关系，以及形态学、生理学和化学分类学特征的数据表明，这两个分离物可以被分类为交替单胞菌属中的两个新种的代表，因此将Alteromonas tagae sp. nov.（模式菌株AT 1（T）= BCRC 17571（T）=JCM 13895（T））和Alteromonas simidui sp. nov.（模式菌株AS 1（T）= BCRC 17572（T）=JCM 13896（T））。
Two mercury-resistant strains of heterotrophic, aerobic, marine bacteria, designated AT 1 T and AS1(T), were isolated from water samples collected from the Er-Jen River estuary, Tainan, Taiwan. Cells were Gram-negative rods that were motile by means of a single polar flagellum. Buds and prosthecae were produced. The two isolates required NaCl for growth and grew optimally at about 30 degrees C, 2-4% NaCl and pH 7-8. They grew aerobically and were incapable of anaerobic growth by fermenting glucose or other carbohydrates. They grew and expressed Hg2+-reducing activity in liquid media containing HgCl2. Strain AS1(T) reduced nitrate to nitrite. The predominant isoprenoid quinone was Q(8) (91.3-99.9%). The polar lipids of strain AT1(T) consisted of phosphaticlylethanolamine (46.6%), phosphatidylglycerol (28.9%) and sulfolipid (24.5%), whereas those of AS1(T) comprised phosphaticlylethanolamine (48.2%) and phosphatidylglycerol (51.8%). The two isolates contained C-16:(1)omega 7c and/or iso-C-15:0 2-OH (22.4-33.7%), C-16:0 (19.0-22.7%) and C-18:1 omega 7c (11.3-11.7%) as the major fatty acids. Strains AT1(T) and AS1(T) had DNA G + C contents of 43.1 and 45.3 mol%, respectively. Phylogeny based on 16S rRNA gene sequences, together with data from morphological, physiological and chemotaxonomic characterization, indicated that the two isolates could be classified as representatives of two novel species in the genus Alteromonas, for which the names Alteromonas tagae sp. nov. (type strain AT1(T) = BCRC 17571(T)=JCM 13895(T)) and Alteromonas simiduii sp. nov. (type strain AS1(T) = BCRC 17572(T)=JCM 13896(T)) are proposed.