The fetal liver counterpart of adult common lymphoid progenitors gives rise to all lymphoid lineages, CD45+CD4+CD3- cells, as well as macrophages
The fetal liver counterpart of adult common lymphoid progenitors gives rise to all lymphoid lineages, CD45+CD4+CD3- cells, as well as macrophages
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DOI：
10.4049/jimmunol.166.11.6593
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发表时间：
2001-06-01
影响因子：
4.4
通讯作者：
Akashi, K
中科院分区：
文献类型：
--
作者：
Mebius, RE;Miyamoto, T;Akashi, K
我们在E14胎肝中鉴定了IL-7 R（α +）Sca-1（低）c-Kit（低）群体，它是成人骨髓中常见淋巴祖细胞（CLP）的表型类似物。转移到新生小鼠后，IL-7 R alpha（+）Sca-1（低）c-Kit（低）群体迅速分化为CD 45（+）CD 4（+）CD 3（-）细胞，这些细胞是启动淋巴结和派伊尔集合淋巴结形成的候选细胞。此外，该群体还产生了B、T、NK和CD 8 α（+）和CD 8 α（-）树突状细胞。胎肝前体细胞表达的骨髓抑制转录因子Pax-5水平显著低于成人CLP，并保留了体外巨噬细胞的分化活性。我们认为，从胎肝IL-7 R α（+）Sca-1（低）Kit（低）细胞到成人CLP的转变涉及其发育潜力的调节限制，至少部分受Pax-5表达的控制。
We identified an IL-7R(alpha+)Sca-1(low)c-Kit(low) population in E14 fetal liver, which is the phenotypical analog of common lymphoid progenitors (CLP) in adult bone marrow. After transfer into newborn mice, the IL-7R alpha (+)Sca-1(low)c-Kit(low) population rapidly differentiated into CD45(+)CD4(+)CD3(-) cells, which are candidate cells for initiating lymph node and Peyer's patch formation. In addition, this population also gave rise to B, T, NK, and CD8 alpha (+) and CD8 alpha (-) dendritic cells. The fetal liver precursors expressed a significantly lower level of the myeloid-suppressing transcription factor Pax-5, than adult CLP, and retained differentiation activity for macrophages in vitro. We propose that the transition from fetal liver IL-7R alpha (+)Sca-1(low)Kit(low) cells to adult CLP involves a regulated restriction of their developmental potential, controlled, at least in part, by Pax-5 expression.