Molecular cloning and characterization of chemokine-like factor 1 (CKLF1), a novel human cytokine with unique structure and potential chemotactic activity
Molecular cloning and characterization of chemokine-like factor 1 (CKLF1), a novel human cytokine with unique structure and potential chemotactic activity
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DOI：
10.1042/0264-6021:3570127
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发表时间：
2001-07-01
影响因子：
4.1
通讯作者：
Ma, DL
中科院分区：
文献类型：
--
作者：
Han, WL;Lou, YX;Ma, DL
细胞因子是在免疫和炎症反应中具有重要作用的小蛋白质。细胞因子的库正变得多样化和扩大。在这里，我们报告了一种新的细胞因子命名为趋化因子样因子1（CKLF 1）的鉴定和表征。CKLF 1基因全长530 bp，开放阅读框编码99个氨基酸残基。CKLF 1的氨基酸序列与其它已知细胞因子无明显相似性。IL 10可部分抑制PHA刺激的U937细胞中CKLF 1的表达。重组CKLF 1是一种有效的中性粒细胞化学引诱剂。单核细胞和淋巴细胞;此外，它可以刺激小鼠骨骼肌细胞的增殖。这些结果表明CKLF 1可能在炎症和骨骼肌再生中起重要作用。
Cytokines are small proteins that have an essential role in the immune and inflammatory responses. The repertoire of cytokines is becoming diverse and expanding. Here we report the identification and characterization of a novel cytokine designated as chemokine-like factor 1 (CKLF1). The full-length cDNA of CKLF1 is 530 bp long and a single open reading frame encoding 99 amino acid residues. CKLF1 bears no significant similarity to any other known cytokine in its amino acid sequence, Expression of CKLF1 can be partly inhibited by interleukin 10 in PHA-stimulated U937 cells. Recombinant CKLF1 is a potent chemoattractant for neutrophils. monocytes and lymphocytes; moreover, it can stimulate the proliferation of murine skeletal muscle cells. These results suggest that CKLF1 might have important roles in inflammation and in the regeneration of skeletal muscle.