Microbial degradation of cyanobacterial cyclic peptides
Microbial degradation of cyanobacterial cyclic peptides
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DOI：
10.1016/j.watres.2007.01.003
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发表时间：
2007-04-01
期刊：
影响因子：
12.8
通讯作者：
Harada, Ken-ichi
中科院分区：
文献类型：
--
作者：
Kato, Hajime;Imanishi, Susumu Y.;Harada, Ken-ichi
细菌菌株 B-9 拥有能够降解微囊藻毒素 (MC) 和结球菌素（蓝细菌产生的有毒环肽）的水解酶。本研究研究了B-9细胞提取物对MCs和节球菌素以外的无毒蓝藻环肽的降解活性，并通过液相色谱/离子阱串联质谱（LC/ITMS）对降解产物进行了分析。经证实，B-9还可以通过水解蓝藻环肽的肽键来降解这些环肽。这些结果表明，该细菌具有非常独特的水解活性，可以降解结构不同的环肽，这可能对有害环肽的解毒有效。 (c) 2007 Elsevier Ltd. 保留所有权利。
Bacterial strain B-9 possesses hydrolytic enzymes capable of degrading microcystins (MCs) and nodularin that are toxic cyclic peptides produced by cyanobacteria. In the present study, the degradation activities of the cell extract of B-9 against non-toxic cyanobacterial cyclic peptides other than the MCs and nodularin were investigated, and the degradation products were analyzed by liquid chromatography/ion trap tandem mass spectrometry (LC/ITMS). It was confirmed that B-9 could also degrade these cyanobacterial cyclic peptides by hydrolysis of their peptide bonds. These results indicated that this bacterium possesses a very unique hydrolytic activity that can degrade structurally different cyclic peptides and that this may be effective for the detoxification of hazardous cyclic peptides. (c) 2007 Elsevier Ltd. All rights reserved.