Analysis of the genomes of mink cell focus-inducing murine type-C viruses: a progress report.
Analysis of the genomes of mink cell focus-inducing murine type-C viruses: a progress report.
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水貂细胞焦点诱导鼠 C 型病毒的基因组分析：进展报告。
DOI：
10.1101/sqb.1980.044.01.138
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Hopkins,N
中科院分区：
文献类型：
--
作者：
Lung,ML;Hering,C;Hartley,JW;Rowe,WP;Hopkins,N
材料和方法细胞和病毒株。先前已经描述了这些研究中使用的MCF病毒的分离（Rowe等人，此卷）。从AKR株的单个白血病小鼠（MCF分离株1375-1、1375-2和勒克-3）或白血病NFS Akv-1同源小鼠（MCF分离株M111 A、M116、CM 78和CM 181）或白血病NFS Akv-2同源小鼠（MCF分离株MB 25、CM 66和CM 74）克隆每种病毒。使用每种病毒慢性感染的SC-1细胞（Hartley和Rowe 1975）制备用于RNase-T1指纹分析的32 p标记病毒RNA。
MATERIALS AND METHODSCells and viral strains. The isolation of the MCF viruses used in these studies has been described previously (Rowe et al., this volume). Each virus was cloned from an individual leukemic mouse of the AKR strain (MCF isolates 1375-1, 1375-2, and leuk-3), or from leukemic NFS Akv-1 congenic mice (MCF isolates MlllA, Ml16, CM78, and CM181), or leukemic NFS Akv-2 congenic mice (MCF isolates MB25, CM66, and CM74). SC-1 cells (Hartley and Rowe 1975) chronically infected with each virus were used to prepare 32p-labeled viral RNA for RNase-T1 fingerprinting.