Virus-specific splicing inhibitor in extracts from cells infected with HIV-1.
Virus-specific splicing inhibitor in extracts from cells infected with HIV-1.
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HIV-1 感染细胞提取物中的病毒特异性剪接抑制剂。
DOI：
10.1126/science.3047873
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Goldenberg,CJ
中科院分区：
文献类型：
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作者：
Gutman,D;Goldenberg,CJ
与大多数其他逆转录病毒不同，人类免疫缺陷病毒1型（HIV-1）编码用于病毒基因表达的反式调节蛋白。本研究显示，通过体外剪接系统，从感染HIV-1的细胞中获得的核提取物含有一种（或多种）因子，该因子特异性抑制包膜蛋白编码区中含有供体和受体剪接位点的合成SP 6/HIV前信使RNA（前mRNA）的剪接。它也表明，SP 6/HIV前mRNA是不能组装在核糖核蛋白复合物，剪接体，从感染的细胞提取物。这些发现提出了这样一种可能性，即HIV-1反式调节因子对包膜蛋白编码区前mRNA剪接的特异性抑制可能是有效生产病毒结构蛋白和病毒粒子组装的一种控制机制。
Human immunodeficiency virus type 1 (HIV-1), in contrast with most other retroviruses, encodes trans-regulatory proteins for virus gene expression. It is shown in this study, by means of an in vitro splicing system, that nuclear extracts obtained from cells infected with HIV-1 contain a factor (or factors) that specifically inhibits splicing of a synthetic SP6/HIV pre-messenger RNA (pre-mRNA)-containing donor and acceptor splice sites in the coding region for the envelope protein. It is also shown that the SP6/HIV pre-mRNA is not capable of assembly in a ribonucleoprotein complex, spliceosome, in extracts from infected cells. These findings raise the possibility that specific inhibition of pre-mRNA splicing in the envelope protein coding region by HIV-1 trans-regulatory factors might be one control mechanism for efficient production of structural viral proteins and virion assembly.