Erythrocyte-based Pig-a gene mutation assay.
Erythrocyte-based Pig-a gene mutation assay.
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基于红细胞的 Pig-a 基因突变测定。
DOI：
10.1007/978-1-62703-529-3_3
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
Dertinger,StephenD
中科院分区：
文献类型：
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作者：
Bemis,JeffreyC;Hall,NikkiE;Dertinger,StephenD
除染色体损伤外，基因突变评估是临床前安全性试验中基因毒性试验的重要组成部分。猪基因突变测定是基于猪基因功能的丧失，导致GPI锚定靶向表面的特定蛋白质缺乏细胞表面表达。这种细胞表面表型很容易通过流式细胞术分析红细胞来评估。本单元描述了使用MutaFlow®试剂盒和普通台式流式细胞仪提供的材料收集，处理和分析外周血样本的程序。
In addition to chromosomal damage, assessment of gene mutation is an important part of genotoxicity testing employed during preclinical safety testing. ThePig-agene mutation assay is based on the loss of function of thePig-agene, which results in a lack of cell surface expression of specific proteins that are targeted to the surface by GPI anchors. This cell surface phenotype is readily assessed by flow cytometric analysis of red blood cells. This unit describes a procedure for the collection, processing, and analysis of peripheral blood samples using materials supplied in MutaFlow®kits and a common benchtop flow cytometer.