LONG-LASTING MORPHOLOGICAL-CHANGES IN DENDRITIC SPINES OF DENTATE GRANULAR CELLS FOLLOWING STIMULATION OF ENTORHINAL AREA
LONG-LASTING MORPHOLOGICAL-CHANGES IN DENDRITIC SPINES OF DENTATE GRANULAR CELLS FOLLOWING STIMULATION OF ENTORHINAL AREA
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DOI：
10.1007/bf01261506
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发表时间：
1977-01-01
期刊：
影响因子：
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通讯作者：
VANHARREVELD, A
中科院分区：
文献类型：
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作者：
FIFKOVA, E;VANHARREVELD, A
刺激[小鼠]穿孔路径会诱导树突棘面积的持久增加，这些区域是齿状分子层远端三分之一的刺激路径的终止位点。没有扩大的棘被发现在近端三分之一的齿状分子层，连合传入终止。以30次/s的速度进行30 s的强直刺激后，在刺激后2-6 min、10-60 min、4-8 h和23 h的时间间隔内，脊髓分别显著增大15%、38%、35%和23%。轴突终末的面积在2-6 min内减少15%，泡密度在10-60 min内减少19%。这两种变化是可逆的，终端恢复其刺激前的条件，在较长的时间间隔（> 4小时）。最初扩大的棘被解释为是由于谷氨酸诱导的增加的Na+渗透性的棘膜，而对于长期的扩大蛋白质合成的增加被假定。树突棘在齿状分子层的持久扩大后，短期列车的刺激传递到perforant路径，支持的假设，这种变化的机制，长期持久的postactivation增强观察到在这条途径。
Stimulation of the [mouse] perforant path induces a long-lasting increase in the area of dendritic spines, which are sites of termination of the stimulated pathway in the distal third of the dentate molecular layer. No enlarged spines were found in the proximal third of the dentate molecular layer, where the commissural afferents terminate. Following a single tetanic stimulus of 30 s duration at 30/s, spines became significantly larger by 15, 38, 35 and 23% within poststimulation intervals of 2-6 min, 10-60 min, 4-8 h and 23 h, respectively. Axon terminals decreased their area by 15% within the 2-6 min interval and the vesicle density was decreased by 19% within the 10-60 min interval. Both changes were reversible and terminals resumed their prestimulation condition at longer intervals (> 4 h). The initial enlargement of spines was interpreted as being due to a glutamate-induced increase in the Na+ permeability of the spine membrane, whereas for the long-lasting enlargement an increase in protein synthesis was postulated. The long-lasting enlargement of dendritic spines in the dentate molecular layer following a short train of stimuli delivered to the perforant path, supports the postulate which links such a change to the mechanism of long-lasting postactivation potentiation observed in this pathway.