Oral administration of human rotavirus to volunteers: induction of illness and correlates of resistance.
Oral administration of human rotavirus to volunteers: induction of illness and correlates of resistance.
复制标题
志愿者口服人轮状病毒：疾病的诱发和耐药性的相关性。
DOI：
10.1093/infdis/147.1.95
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
R. Chanock
中科院分区：
文献类型：
--
作者：
A. Kapikian;R. Wyatt;M. Levine;R. Yolken;D. Vankirk;R. Dolin;H. Greenberg;R. Chanock
口服人类轮状病毒D株(亚群2，WA型)的18名志愿者中，有4人在接种后2-4天出现腹泻病。在18名志愿者中有5人检测到病毒脱落，而12人(67%)出现了感染的血清学证据。两名在最初接种后出现腹泻疾病的志愿者在19个月后接受了相同的接种；两人都没有出现腹泻，尽管其中一人出现了体质和胃肠道症状。接种前血清中抗轮状病毒D株免疫荧光抗体或高水平中和抗体Wa或重组人轮状病毒DS-1与对腹泻病的抵抗力相关。虽然攻击前血清抗体与抵抗腹泻和/或轮状病毒脱落有关，但肠液中预先存在的局部中和活性之间的关系不太明确。
Four of 18 volunteers challenged orally with human rotavirus strain D (subgroup 2, serotype Wa) developed a diarrheal illness two to four days after inoculation. Viral shedding was detected in five of the 18 volunteers, whereas 12 (67%) developed serologic evidence of infection. Two volunteers who developed diarrheal illness after the initial inoculation were given the same inoculum 19 months later; neither developed diarrhea, although one developed constitutional and gastrointestinal symptoms. The presence of preinoculation serum immunofluorescent antibody to rotavirus strain D or high levels of neutralizing antibody to Wa or reassortant DS-1 human rotavirus correlated with resistance to diarrheal illness. Although prechallenge serum antibody correlated with resistance to diarrhea and/or shedding of rotavirus, the relationship of preexisting local neutralizing activity in intestinal fluid was less clear-cut.