INDUCTION OF THE HUMAN GENE FOR P44, A HEPATITIS-C-ASSOCIATED MICROTUBULAR AGGREGATE PROTEIN, BY INTERFERON-ALPHA/BETA
INDUCTION OF THE HUMAN GENE FOR P44, A HEPATITIS-C-ASSOCIATED MICROTUBULAR AGGREGATE PROTEIN, BY INTERFERON-ALPHA/BETA
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DOI：
10.1111/j.1432-1033.1994.00877.x
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发表时间：
1994-09-15
期刊：
影响因子：
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通讯作者：
KITAMURA, N
中科院分区：
文献类型：
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作者：
KITAMURA, A;TAKAHASHI, K;KITAMURA, N
丙型肝炎相关的微管聚集蛋白，称为p44，已被确定为丙型肝炎病毒感染的黑猩猩肝细胞中的细胞质抗原。在感染丙型肝炎或丁型肝炎病毒的黑猩猩的肝脏中，p44 mRNA的产生被显著诱导。为了研究这种诱导的机制，我们分离了人类p44蛋白的基因组克隆并分析了其结构。人类p44基因跨越约14 kbp的DNA，由9个外显子和8个内含子组成。在该基因的启动子区发现了一个干扰素刺激的反应元件，该元件赋予干扰素-α/β的诱导作用。Northern-blot分析显示，人p44基因可被干扰素α/β诱导，但不能被干扰素γ诱导。功能分析表明，在基因的启动子区域中的干扰素刺激的反应元件介导的干扰素α/β的基因的诱导。因此，人p44基因是干扰素-α/β家族的成员。诱导基因。p44蛋白可能是干扰素抗病毒作用的介质之一。
A hepatitis-C-associated microtubular aggregate protein, referred to as p44, has been identified as a cytoplasmic antigen in the hepatocytes of chimpanzees infected with hepatitis C virus. The production of p44 mRNA is markedly induced in the liver of chimpanzees infected with hepatitis C or hepatitis D virus. To examine the mechanism of this induction, we isolated a genomic clone for the human p44 protein and analyzed its structure. The human p44 gene spans approximately 14 kbp of DNA and consists of nine exons separated by eight introns. An interferon-stimulated response element, which confers inducibility by interferon-alpha/beta, was found in the promoter region of the gene. Northern-blot analysis revealed that the human p44 gene is inducible by interferon-alpha/beta,, but not by interferon-gamma. Functional analysis demonstrated that the interferon-stimulated response element in the promoter region of the gene mediates the inducibility of the gene by interferon-alpha/beta. Thus, the human p44 gene is a member of the family of interferon-alpha/beta. inducible genes. The protein p44 may be one of the mediators involved in the antiviral action of interferon.