Genes Galore: A Summary of Methods for Accessing Results from Large-Scale Partial Sequencing of Anonymous Arabidopsis cDNA Clones
Genes Galore: A Summary of Methods for Accessing Results from Large-Scale Partial Sequencing of Anonymous Arabidopsis cDNA Clones
复制标题
Genes Galore：获取匿名拟南芥 cDNA 克隆大规模部分测序结果的方法摘要
作者：
T. Newman;Frans J. de Bruijn;P. Green;K. Keegstra;H. Kende;L. Mclntosh;J. Ohlrogge;N. Raikhel;S. Somerville;M. Thomashow;Ernie Retzel;C. Somerville
匿名cDNA克隆的高通量自动化部分测序提供了一种调查生物体表达基因库的方法。这些表达序列标签（EST）的编码能力与公共数据库中的序列的比较结果在推定的功能分配到一个显着比例的EST。因此，现有的13，400多个植物EST提供了一个新的资源，将促进植物生物学许多领域的进展。这些机会说明了从1500拟南芥EST的cDNA文库制备的多聚（A+）从黄化苗，根，叶和开花花序的mRNA的等份分析获得的结果的描述。超过900个不同的序列，其中32%显示出显着的核苷酸或推导的氨基酸序列相似性，以前表征的基因或蛋白质从广泛的生物体。至少有165个克隆具有显着的推导的氨基酸序列同源性的蛋白质或基因产物，以前没有从高等植物的特点。提供了获取拟南芥cDNA测序项目产生的信息和材料的方法总结。
High-throughput automated partial sequencing of anonymous cDNA clones provides a method to survey the repertoire of expressed genes from an organism. Comparison of the coding capacity of these expressed sequence tags (ESTs) with the sequences in the public data bases results in assignment of putative function to a significant proportion of the ESTs. Thus, the more than 13,400 plant ESTs that are currently available provide a new resource that will facilitate progress in many areas of plant biology. These opportunities are illustrated by a description of the results obtained from analysis of 1500 Arabidopsis ESTs from a cDNA library prepared from equal portions of poly(A+) mRNA from etiolated seedlings, roots, leaves, and flowering inflorescences. More than 900 different sequences were represented, 32% of which showed significant nucleotide or deduced amino acid sequence similarity to previously characterized genes or proteins from a wide range of organisms. At least 165 of the clones had significant deduced amino acid sequence homology to proteins or gene products that have not been previously characterized from higher plants. A summary of methods for accessing the information and materials generated by the Arabidopsis cDNA sequencing projects is provided.
反平行质粒-质粒配对可以控制 P1 质粒复制。
DOI：
10.1073/pnas.88.20.9011
发表时间：
1991
影响因子：
11.1
作者：
Abeles,AL;Austin,SJ
通讯作者：
Austin,SJ
影响因子：
11.6
作者：
LAST, RL;BISSINGER, PH;FINK, GR
通讯作者：
FINK, GR
用于消减杂交、融合蛋白合成和 Cre-loxP 自动质粒亚克隆的噬菌体 lambda cDNA 克隆载体。
影响因子：
3.5
作者：
Palazzolo,MJ;Hamilton,BA;Ding,DL;Martin,CH;Mead,DA;Mierendorf,RC;Raghavan,KV;Meyerowitz,EM;Lipshitz,HD
通讯作者：
Lipshitz,HD