Vernalization can regulate flowering time through microRNA mechanism in Brassica rapa
Vernalization can regulate flowering time through microRNA mechanism in Brassica rapa
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DOI：
10.1111/ppl.12692
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发表时间：
2018-10-01
影响因子：
6.4
通讯作者：
Liu, Tongkun
中科院分区：
文献类型：
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作者：
Huang, Feiyi;Wu, Xiaoting;Liu, Tongkun
春化是调节植物花转变的重要过程。 MicroRNA (miRNA) 是内源性非编码小 RNA (sRNA) 分子，在植物生长和发育中发挥作用。尽管之前已经对与开花相关的 miRNA 进行了表征，但它们在小菜（Brassica rapa ssp. chinensis）春化反应中的作用从未被研究过。在此，构建了来自白菜叶（春化植物和非春化植物）的两个 sRNA 文库并进行了测序。获得了208个已知的miRNA和535个新的miRNA，其中20个已知的miRNA和66个新的miRNA表达显着差异，被认为是春化相关的miRNA。在序列同源性搜索的基础上预测相应的靶标。此外，还选择了 11 个 miRNA 和 8 个靶标进行实时定量 PCR，以确认其表达谱。使用基因本体论和京都基因百科全书和基因组结果对靶标进行功能注释表明，大多数靶标在激素信号通路中显着富集。此外，春化后检测到吲哚-3-乙酸（IAA）减少和GA（3）激素增加，表明IAA和GA（3）可能对春化有反应。这些结果表明白菜春化通过microRNA机制通过影响内源激素水平来调节开花。这项研究提供了参与白菜春化的有希望的 miRNA 候选者的有用见解，并有助于进一步研究 miRNA 介导的白菜春化分子机制。
Vernalization is an important process that regulates the floral transition in plants. MicroRNAs (miRNAs) are endogenous non-coding small RNA (sRNA) molecules that function in plant growth and development. Despite that miRNAs related to flowering have previously been characterized, their roles in response to vernalization in pak-choi (Brassica rapa ssp. chinensis) has never been studied. Here, two sRNA libraries from B. rapa leaves (vernalized and non-vernalized plants) were constructed and sequenced. Two hundred eight known and 535 novel miRNAs were obtained, of which 20 known and 66 new miRNAs were significantly differentially expressed and considered as vernalization-related miRNAs. The corresponding targets were predicted on the basic of sequence homology search. In addition, 11 miRNAs and eight targets were selected for real-time quantitative PCR to confirm their expression profiles. Functional annotation of targets using gene ontology and Kyoto encyclopedia of genes and genomes results suggested that most targets were significantly enriched in the hormone signaling pathway. Moreover, a decreased indole-3-acetic acid (IAA) and an increased GA(3) hormone were detected after vernalization, indicating that the IAA and GA(3) might response to vernalization. These results indicated that vernalization regulates flowering through microRNA mechanism by affecting endogenous hormone level in B. rapa. This study provides useful insights of promising miRNAs candidates involved in vernalization in B. rapa, and facilitates further investigation of the miRNA-mediated molecular mechanisms of vernalization in B. rapa.