Solanum lycopersicum microRNA1916 targets multiple target genes and negatively regulates the immune response in tomato
Solanum lycopersicum microRNA1916 targets multiple target genes and negatively regulates the immune response in tomato
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番茄 microRNA1916 靶向多个靶基因并负向调节番茄的免疫反应
DOI：
10.1111/pce.13468
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发表时间：
2019-04-01
影响因子：
7.3
通讯作者：
Luan, Yu Shi
中科院分区：
文献类型：
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作者：
Chen, Lei;Meng, Jun;Luan, Yu Shi
MicroRNA1916(MiR1916)是植物中响应各种逆境的非保守miRNAs之一，但目前对其在生物逆境中的作用机制知之甚少。本研究发现番茄侵染马铃薯晚疫病菌或番茄灰霉病菌后，番茄中茄子miR1916基因的表达下调。过量表达Sly-miR1916的番茄植株对致病疫霉和灰霉病菌的敏感性显著增强，并增加了产生活性氧的倾向。通过短串联靶标模拟和人工microRNA策略沉默sly-miR1916使番茄植株对逆境更加耐受。此外，Sly-miR1916的低表达还可以上调严格糖苷合成酶(STR-2)、UDP糖基转移酶(UGTS)、晚疫病抗性蛋白同系物R1b-16、抗病蛋白RPP13样蛋白和MYB转录因子(MYB12)的表达，最终通过STR-2、UGT和MYB12导致α-番茄红素和花青素的积累。此外，Sly-miR1916/STR-2的异源表达显著改变了烟草对灰霉病的耐性。综上所述，Sly-miR1916可能通过调控番茄植株中STR-2、UGT和MYB12的表达，通过调节α-番茄红素和花青素来提高对生物胁迫的敏感性。
MicroRNA1916 (miR1916) is one of the nonconserved miRNAs that respond to various stresses in plants, but little has been known at present about its mechanisms in biotic stresses. In this study, the expression of Solanum lycopersicum (sly)-miR1916 in tomato was found to be down-regulated after infection with Phytophthora infestans or Botrytis cinerea. Tomato plants that overexpressed sly-miR1916 displayed significant enhancement in susceptibility to P. infestans and B. cinerea infection, as well as increased tendency to produce reactive oxygen species. Silencing of sly-miR1916 by short tandem target mimic and artificial microRNA strategies caused the tomato plants to become more tolerant to adverse conditions. In addition, lower sly-miR1916 expression could up-regulate the expression of strictosidine synthase (STR-2), UDP-glycosyltransferases (UGTs), late blight resistance protein homolog R1B-16, disease resistance protein RPP13-like, and MYB transcription factor (MYB12), which ultimately resulted in the accumulation of alpha-tomatine and anthocyanins via STR-2, UGT, and MYB12. Furthermore, ectopic expression of sly-miR1916/STR-2 significantly changed the tolerance of tobacco to B. cinerea. Taken together, the results demonstrated that sly-miR1916 might regulate the expression of STR-2, UGT, and MYB12 in tomato plant, conferring sensitivity to biotic stress via modulating alpha-tomatine and anthocyanins.