CONFORMATION AND MOTION OF CHOLINE HEAD GROUP IN BILAYERS OF DIPALMITOYL-3-SN-PHOSPHATIDYLCHOLINE
CONFORMATION AND MOTION OF CHOLINE HEAD GROUP IN BILAYERS OF DIPALMITOYL-3-SN-PHOSPHATIDYLCHOLINE
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DOI：
10.1021/bi00687a021
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发表时间：
1975-01-01
期刊：
影响因子：
2.9
通讯作者：
SEELIG, J
中科院分区：
文献类型：
--
作者：
GALLY, HU;NIEDERBERGER, W;SEELIG, J
Hans-Ulrich Gyndham，Werner Niederberger和Joachim Seelig* 摘要：通过氘和磷磁共振研究了凝胶-液晶转变点以上和以下脂质双层中胆碱头部基团的构象和运动。为此目的，二棕榈酰-3-异磷脂酰胆碱在胆碱和甘油成分上的不同位置被选择性氘代。剩余的氘核四极耦合和磷化学位移各向异性相应的脂水混合物产生的节段运动的定量信息。胆碱甲基的运动仅受到轻微的阻碍，但运动自由度随着该片段离甘油主链越近而受到越来越大的限制。OC-CN键旋转角的平均值随温度的变化而变化。在-
Hans-Ulrich Gaily, Werner Niederberger, and Joachim Seelig* abstract: The conformation and motion of the choline head group in lipid bilayers above and below the gel-to-liquid crystal transition point are studied by means of deuteri-um and phosphorus magnetic resonance. For this purpose dipalmitoyl-3-i «-phosphatidylcholine is selectively deuter-ated at various positions on the choline and glycerol constit-uents. The residual deuteron quadrupole couplings and the phosphorus chemical-shift anisotropy of the corresponding lipid-water mixtures yield quantitative information on the segmental motions. The choline methyl group is only slight-ly hindered in its movement, but the motional freedom be-comes increasingly restricted the closer the segment is lo-cated to the glycerol backbone. The average value of the OC-CN bond rotation angle changes with temperature. In-