The bHLH protein NEUROGENIN 2 is a determination factor for epibranchial placode-derived sensory neurons
The bHLH protein NEUROGENIN 2 is a determination factor for epibranchial placode-derived sensory neurons
复制标题
DOI：
10.1016/s0896-6273(00)80989-7
复制
发表时间：
1998-03-01
期刊：
影响因子：
16.2
通讯作者：
Guillemot, F
中科院分区：
文献类型：
--
作者：
Fode, C;Gradwohl, G;Guillemot, F
neurogenin2编码与果蝇前神经因子atonal相关的神经特异性基本螺旋-环-螺旋（bHLH）转录因子。我们在这里表明，小鼠ngn2基因对鳃外基板源性颅感觉神经节的发育至关重要。ngn2缺失突变阻断了来自基板的神经元前体的分层，这是这些谱系中分化的第一个形态学标志。突变的胎盘细胞不能表达下游bHLH分化因子和Notch配体Delta-like 1。这些数据表明，ngn2在决定远端颅神经节神经元命运方面的功能与果蝇前神经基因类似。有趣的是，同源盒基因Phox2a在鳃外基板中独立于ngn2被激活，这表明神经元的命运和神经元亚型身份可能在颅感觉神经节中独立被指定。
neurogenin2 encodes a neural-specific basic helix-loop-helix (bHLH) transcription factor related to the Drosophila proneural factor atonal. We show here that the murine ngn2 gene is essential for development of the epibranchial placode-derived cranial sensory ganglia. An ngn2 null mutation blocks the delamination of neuronal precursors from the placodes, the first morphological sign of differentiation in these lineages. Mutant placodal cells fail to express downstream bHLH differentiation factors and the Notch ligand Delta-like 1. These data suggest that ngn2 functions like the Drosophila proneural genes in the determination of neuronal fate in distal cranial ganglia. Interestingly, the homeobox gene Phox2a is activated independently of ngn2 in epibranchial placodes, suggesting that neuronal fate and neuronal subtype identity may be specified independently in cranial sensory ganglia.