Comparative Transcriptome Analysis Reveals Different Silk Yields of Two Silkworm Strains.
Comparative Transcriptome Analysis Reveals Different Silk Yields of Two Silkworm Strains.
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比较转录组分析揭示了两种蚕品系的不同丝产量
DOI：
10.1371/journal.pone.0155329
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Li M
中科院分区：
文献类型：
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作者：
Li J;Qin S;Yu H;Zhang J;Liu N;Yu Y;Hou C;Li M
茧丝产量是蚕桑业最重要的特征。然而，很少有研究检查调节这些特征的基因。对这些基因的进一步研究将有助于改善蚕桑产品。 JingSong（JS）和Lan10（L10）是两个茧丝产量差异显着的品系。在当前的研究中，对两种菌株进行了RNA测序和定量聚合酶链反应（qPCR），以确定丝腺的分化，丝腺控制着蚕的丝生物合成。与L10相比，JS有1375个差异表达基因（DEG；738个上调基因和673个下调基因）。基于这些 DEG，通过 GO 富集分析确定了 9 个富集基因本体 (GO) 术语。 KEGG富集分析结果显示DEG在3条通路上富集，主要与蛋白质的加工和生物合成有关。通过qPCR对富集途径中的代表性基因和10个显着DEG进行进一步验证，结果与RNA-Seq数据一致。我们的研究揭示了两种蚕品系之间丝腺的差异，并为理解决定丝产量的分子机制提供了视角。
Cocoon and silk yields are the most important characteristics of sericulture. However, few studies have examined the genes that modulate these features. Further studies of these genes will be useful for improving the products of sericulture. JingSong (JS) and Lan10 (L10) are two strains having significantly different cocoon and silk yields. In the current study, RNA-Seq and quantitative polymerase chain reaction (qPCR) were performed on both strains in order to determine divergence of the silk gland, which controls silk biosynthesis in silkworms. Compared with L10, JS had 1375 differentially expressed genes (DEGs; 738 up-regulated genes and 673 down-regulated genes). Nine enriched gene ontology (GO) terms were identified by GO enrichment analysis based on these DEGs. KEGG enrichment analysis results showed that the DEGs were enriched in three pathways, which were mainly associated with the processing and biosynthesis of proteins. The representative genes in the enrichment pathways and ten significant DEGs were further verified by qPCR, the results of which were consistent with the RNA-Seq data. Our study has revealed differences in silk glands between the two silkworm strains and provides a perspective for understanding the molecular mechanisms determining silk yield.