Concurrent purification and antioxidant activity of phycobiliproteins from Lyngbya sp A09DM: An antioxidant and anti-aging potential of phycoerythrin in Caenorhabditis elegans
Concurrent purification and antioxidant activity of phycobiliproteins from Lyngbya sp A09DM: An antioxidant and anti-aging potential of phycoerythrin in Caenorhabditis elegans
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DOI：
10.1016/j.procbio.2014.06.022
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发表时间：
2014-10-01
影响因子：
4.4
通讯作者：
Madamwar, Datta
中科院分区：
文献类型：
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作者：
Sonani, Ravi Raghav;Singh, Niraj Kumar;Madamwar, Datta
本研究探讨了藻胆蛋白的分离纯化及其体外抗氧化活性。此外，该研究还证明了藻红蛋白的抗氧化作用在减缓秀丽隐杆线虫衰老现象中的作用。采用硫酸铵分级沉淀结合TritonX-100层析的方法，从鞘丝藻（Lyngbya sp.A09DM）中成功地分离纯化了藻红蛋白、藻蓝蛋白和别藻蓝蛋白。通过一系列生物化学表征如SDS-PAGE、非变性PAGE、UV-可见光谱和荧光光谱检查方案的成功，确保纯化的藻红蛋白、藻蓝蛋白和别藻蓝蛋白的纯度、完整性和功能性。纯化的藻胆蛋白的抗氧化剂和金属离子螯合活性进行了评估，通过各种体外抗氧化剂测定系统。结果表明，所有藻胆蛋白的抗氧化能力和金属螯合能力均具有明显的剂量依赖性，其顺序为藻红蛋白>藻蓝蛋白>别藻蓝蛋白。延长C.的寿命并改善其咽泵功能。在用藻红蛋白（100 μ g/ml）预处理后观察到秀丽线虫。此外，藻红蛋白介导的增加蠕虫在氧化应激下的存活揭示了藻红蛋白对线虫的生命扩展效应部分是通过其抗氧化剂美德的作用。这些结果共同增加了支持“衰老的自由基理论”的证据。本文首次报道了藻红蛋白的抗氧化能力及其在延长C.优雅的。(C)2014爱思唯尔有限公司版权所有。
The present study probes into the purification of phycobiliproteins, and characterization of their in vitro anti-oxidant activity. Moreover, the study also demonstrates the use of antioxidant virtue of phycoerythrin in moderating the phenomenon of aging in Caenorhabditis elegans. Phycoerythrin, phycocyanin and allophycocyanin were purified successfully from Lyngbya sp. A09DM by ammonium sulfate fractionation appended with Triton X-100 intercession. The success of protocol was examined by a series of biochemical characterization like SDS-PAGE, native-PAGE, UV-visible spectroscopy and fluorescence spectroscopy ensuring purity, integrity and functionality of purified phycoerythrin, phycocyanin and allophycocyanin. Purified phycobiliproteins were evaluated for antioxidant and metal ion chelating activity by various in vitro antioxidant assay systems. Results showed significant and dose-dependent antioxidant as well as metal chelating potential of all phycobiliproteins in decreasing order of phycoerythrin > phycocyanin > allophycocyanin. Expansion in lifespan and improvement in pharyngeal pumping of C. elegans were noticed upon pre-treatment with phycoerythrin (100 mu g ml(-1)). Moreover, phycoerythrin mediated increase in worm survival under oxidative stress revealed that the life expansion effect of phycoerythrin on nematode is in part by an action of its antioxidant virtue. These results collectively added up evidence in favor of the 'free-radical theory of aging'. The present report, for the first time, describes antioxidant potential of phycoerythrin and its use in extending life-span of C. elegans. (C) 2014 Elsevier Ltd. All rights reserved.