Legionella pneumophila utilizes a single-player disulfide-bond oxidoreductase system to manage disulfide bond formation and isomerization.
Legionella pneumophila utilizes a single-player disulfide-bond oxidoreductase system to manage disulfide bond formation and isomerization.
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嗜肺军团菌利用单机二硫键氧化还原酶系统来管理二硫键的形成和异构化。
DOI：
10.1111/mmi.12914
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发表时间：
2015-03
影响因子：
3.6
通讯作者：
Hoffman PS
中科院分区：
文献类型：
--
作者：
Kpadeh ZZ;Day SR;Mills BW;Hoffman PS
Legionella pneumophila uses a single homodimeric disulfide bond (DSB) oxidoreductase DsbA2 to catalyze extracytoplasmic protein folding and to correct DSB errors through protein-disulfide isomerase (PDI) activity. In Escherichia coli, these functions are separated to avoid futile cycling. In L. pneumophila, DsbA2 is maintained as a mixture of disulfides (S-S) and free thiols (SH), but when expressed in E. coli, only the SH form is observed. We provide evidence to suggest that structural differences in DsbB oxidases (LpDsbB1 and LpDsbB2) and DsbD reductases (LpDsbD1 and LpDsbD2) (compared to E. coli) permit bifunctional activities without creating a futile cycle. LpdsbB1 and LpdsbB2 partially complemented an EcdsbB mutant while neither LpdsbD1 nor LpdsbD2 complemented an EcdsbD mutant unless DsbA2 was also expressed. When the dsb genes of E. coli were replaced with those of L. pneumophila, motility was restored and DsbA2 was present as a mixture of redox forms. A dominant-negative approach to interfere with DsbA2 function in L. pneumophila determined that DSB oxidase activity was necessary for intracellular multiplication and assembly/function of the Dot/Icm Type IVb secretion system. Our studies show that a single-player system may escape the futile cycle trap by limiting transfer of reducing equivalents from LpDsbDs to DsbA2.
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影响因子：
4.4
作者：
Ewann, Fanny;Hoffman, Paul S.
通讯作者：
Hoffman, Paul S.
DOI：
10.1073/pnas.90.3.1043
发表时间：
1993-02-01
影响因子：
11.1
作者：
DAILEY, FE;BERG, HC
通讯作者：
BERG, HC
影响因子：
4.8
作者：
Cho, Seung-Hyun;Beckwith, Jon
通讯作者：
Beckwith, Jon
影响因子：
11.4
作者：
Bader, MW;Hiniker, A;Bardwell, JCA
通讯作者：
Bardwell, JCA
影响因子：
6.4
作者：
Cho, Seung-Hyun;Parsonage, Derek;Beckwith, Jon
通讯作者：
Beckwith, Jon