Using an isolated rat kidney model to identify kidney origin proteins in urine.
Using an isolated rat kidney model to identify kidney origin proteins in urine.
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DOI：
10.1371/journal.pone.0066911
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Gao Y
中科院分区：
文献类型：
--
作者：
Jia L;Li X;Shao C;Wei L;Li M;Guo Z;Liu Z;Gao Y
利用靶向蛋白质组学技术在尿液中鉴定肾脏疾病的尿液生物标志物，可以避免血清蛋白的干扰。它可以提供更好的样品通量、更高的灵敏度和特异性。了解哪些尿蛋白是目标是必不可少的。通过对离体大鼠肾脏灌流后尿液的分析，鉴定出990个与人同源的肾源蛋白。在这些蛋白质中，有128种在正常人尿液中未发现，可能成为零背景的生物标志物。在正常人血浆中未发现297种蛋白质。这些蛋白质不会受到其他正常器官的影响，并且是肾脏特异性的。33种蛋白质的水平增加灌注期间与缺氧的解决方案相比，那些灌注氧气。灌注驱动尿液中的75种蛋白质与其在正常人尿液中的排序相比具有显著增加的丰度排序。与现有的候选生物标志物相比，本研究中鉴定的尿液中超过90%的肾源性蛋白尚未被检查为肾脏疾病的候选生物标志物。
The use of targeted proteomics to identify urinary biomarkers of kidney disease in urine can avoid the interference of serum proteins. It may provide better sample throughput, higher sensitivity, and specificity. Knowing which urinary proteins to target is essential. By analyzing the urine from perfused isolated rat kidneys, 990 kidney origin proteins with human analogs were identified in urine. Of these proteins, 128 were not found in normal human urine and may become biomarkers with zero background. A total of 297 proteins were not found in normal human plasma. These proteins will not be influenced by other normal organs and will be kidney specific. The levels of 33 proteins increased during perfusion with an oxygen-deficient solution compared to those perfused with oxygen. The 75 proteins in the perfusion-driven urine have a significantly increased abundance ranking compared to their ranking in normal human urine. When compared with existing candidate biomarkers, over ninety percent of the kidney origin proteins in urine identified in this study have not been examined as candidate biomarkers of kidney diseases.
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影响因子：
14.9
作者：
NCBI Resource Coordinators
通讯作者：
NCBI Resource Coordinators
影响因子：
2.7
作者：
Saha S;Harrison SH;Shen C;Tang H;Radivojac P;Arnold RJ;Zhang X;Chen JY
通讯作者：
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影响因子：
7
作者：
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通讯作者：
Birney, Ewan
影响因子：
13.6
作者：
Gonzales, Patricia A.;Pisitkun, Trairak;Knepper, Mark A.
通讯作者：
Knepper, Mark A.
影响因子：
7
作者：
Ishihama, Y;Oda, Y;Mann, M
通讯作者：
Mann, M