Acetylation regulates monopolar attachment at multiple levels during meiosis I in fission yeast
Acetylation regulates monopolar attachment at multiple levels during meiosis I in fission yeast
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DOI：
10.1038/embor.2011.188
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发表时间：
2011-11-01
期刊：
影响因子：
7.7
通讯作者：
Watanabe, Yoshinori
中科院分区：
文献类型：
--
作者：
Kagami, Ayano;Sakuno, Takeshi;Watanabe, Yoshinori
在裂殖酵母中，姐妹动粒的减数分裂单一取向是通过在核心着丝粒处的内聚来建立的，该内聚是由减数分裂内聚素复合物和动粒蛋白Moa 1建立的。粘附素亚基Psm 3被Eso 1乙酰化并被Clr 6脱乙酰化。我们发现，在减数分裂，Eso 1是必需的建立核心着丝粒凝聚力在S期，而Moa 1是必需的S期后保持这种凝聚力。clr 6 -1突变抑制moa 1 δ细胞的单取向缺陷，尽管Clr 6抑制的靶点不是Psm 3。因此，几个乙酰化是至关重要的建立和维持核心着丝粒的凝聚力。
In fission yeast, meiotic mono-orientation of sister kinetochores is established by cohesion at the core centromere, which is established by a meiotic cohesin complex and the kinetochore protein Moa1. The cohesin subunit Psm3 is acetylated by Eso1 and deacetylated by Clr6. We show that in meiosis, Eso1 is required for establishing core centromere cohesion during S phase, whereas Moa1 is required for maintaining this cohesion after S phase. The clr6-1 mutation suppresses the mono-orientation defect of moa1 Delta cells, although the Clr6 target for this suppression is not Psm3. Thus, several acetylations are crucial for establishing and maintaining core centromere cohesion.