Mutations in bone morphogenetic protein 15 and growth differentiation factor 9 genes are associated with increased litter size in fat-tailed sheep breeds
Mutations in bone morphogenetic protein 15 and growth differentiation factor 9 genes are associated with increased litter size in fat-tailed sheep breeds
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DOI：
10.1007/s11259-011-9467-9
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发表时间：
2011-03-01
影响因子：
2.2
通讯作者：
Esmailizadeh, Ali K.
中科院分区：
文献类型：
--
作者：
Javanmard, Arash;Azadzadeh, N.;Esmailizadeh, Ali K.
本研究的目的是探讨肥尾羊 GDF9 和 BMP15 基因多态性与产羔数之间的关联，通过 PCR-RFLP 对来自 4 个品种（Afshari=19；Baluchi=18；Makui=30 和 Mehraban=30）的总共 97 只成熟母羊进行了 BMP15 HinfI 和 GDF9 HhaI 多态性基因分型。 技术。 BMP15 基因突变等位基因频率最高和最低的是 Makui (0.27) 和 Afshari (0.10) 羊，GDF9 基因 Afshari (0.24) 和 Mehraban (0.18) 羊中突变等位基因频率最低。产羔数显着受母羊两个基因的基因型影响（P < 0.01）。两个基因座的杂合基因型的窝产仔数均高于纯合基因型（P < 0.01）。这些品种中没有一个个体携带 GDF9 和 BMP15 变体的纯合基因型。携带所研究的候选基因之一的突变等位基因的个体仍然表现出可育表型。因此，BMP15 和 GDF9 变体之一的纯合性的存在可能无法阻断肥尾羊的正常生殖激素途径。
The objective of this study was to investigate association between GDF9 and BMP15 gene polymorphism and litter size in fat-tailed sheep, a total of 97 mature ewes from four breeds (Afshari=19; Baluchi=18; Makui=30 and Mehraban=30) were genotyped for the BMP15 HinfI and GDF9 HhaI polymorphisms by PCR-RFLP technique. The highest and lowest mutant allele frequencies were found in Makui (0.27) and Afshari (0.10) sheep for the BMP15 gene and in Afshari (0.24) and Mehraban (0.18) sheep for the GDF9 gene, respectively. Litter size was significantly influenced by genotype of the ewe for two genes (P < 0.01). Heterozygous genotypes for both loci showed higher litter size than homozygous genotypes (P < 0.01). None of the individuals carried homozygous genotype for both of the GDF9 and BMP15 variants in these breeds. The individuals carrying the mutant allele for one of the investigated candidate gene still showed fertile phenotype. Thus, existence of homozygosity at one of the BMP15 and GDF9 variant is not probably able to block normal hormonal pathway of reproduction in fat-tailed sheep.