Effects of heat stress on filamentous actin and prestin of outer hair cells in mice
Effects of heat stress on filamentous actin and prestin of outer hair cells in mice
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DOI：
10.1016/j.brainres.2007.08.019
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发表时间：
2007-10-26
期刊：
影响因子：
2.9
通讯作者：
Wada, Hiroshi
中科院分区：
文献类型：
--
作者：
Kitsunai, Yoko;Yoshida, Naohiro;Wada, Hiroshi
当耳朵暴露在创伤性噪音中时，外毛细胞（OHC）会受损，从而导致永久性听力损失。最近，据报道，预先进行热应激调节可以通过增加 OHC 体的硬度来保护 OHC 免受创伤性噪声暴露，并且还据报道可以增强畸变产物耳声发射 [DPOAEs； Murakoshi, M.、Yoshida, N.、Kitsunai, Y.、Iida, K.、Kumano, S.、Suzuki, T.、Kobayashi, T.、Wada, H., 2006。热应激对小鼠外毛细胞杨氏模量的影响。脑研究。 1107 121-130]。在本研究中，为了进一步研究热应激诱导的听力保护机制和应激诱导的DPOAE增强机制，对有和没有热应激的OHCs中与细胞硬度有关的丝状肌动蛋白（F-actin）的量和与DPOAE的产生有关的prestin的量进行了检测。研究发现，热应激后 6-24 小时，F-肌动蛋白的量会增加。在角质板处观察到 F-肌动蛋白数量的最大增加，其中 F-肌动蛋白将静纤毛的根锚定到细胞体。基于该结果，认为静纤毛中受热应激最强化和保护的部分是静纤毛的根部。与 F-肌动蛋白相比，热应激不会影响 prestin 的量。 (C) 2007 Elsevier B.V. 保留所有权利。
When the ear is exposed to traumatic loud noise, outer hair cells (OHCs) are damaged and thus permanent hearing loss occurs. Recently, prior conditioning with heat stress has been reported to protect OHCs from traumatic noise exposure by increasing the stiffness of the OHC soma and has also been reported to enhance distortion product otoacoustic emissions [DPOAEs; Murakoshi, M., Yoshida, N., Kitsunai, Y., Iida, K., Kumano, S., Suzuki, T., Kobayashi, T., Wada, H., 2006. Effects of heat stress on Young's modulus of outer hair cells in mice. Brain Res. 1107 121-130]. In the present study, to further investigate the heat stress-induced protective mechanism of hearing and such stress-induced DPOAE enhancement mechanism, the amount of filamentous actin (F-actin), which is concerned with cell stiffness, and the amount of prestin, which is concerned with the generation of DPOAEs, were examined in OHCs, with and without heat stress. Heat stress was found to increase the amount of F-actin 6-24 h after heat stress. The greatest increase in the amount of F-actin was observed at the cuticular plate where F-actin anchors the roots of the stereocilia to the cell body. Based on this result, the part of the stereocilia most reinforced and protected by heat stress was concluded to be the roots of the stereocilia. In contrast with F-actin, heat stress did not affect the amount of prestin. (C) 2007 Elsevier B.V. All rights reserved.