Optimization of spectral-domain optical coherence tomography with a supercontinuum source for in vivo motion detection of low reflective outer hair cells in guinea pig cochleae
Optimization of spectral-domain optical coherence tomography with a supercontinuum source for in vivo motion detection of low reflective outer hair cells in guinea pig cochleae
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DOI：
10.1007/s10043-021-00654-8
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发表时间：
2021-03-29
期刊：
影响因子：
1.2
通讯作者：
Hibino，Hiroshi
中科院分区：
文献类型：
--
作者：
Nin，Fumiaki;Choi，Samuel;Hibino，Hiroshi
声音在感觉上皮细胞内引起亚纳米级的振动。上皮细胞中不仅含有不动细胞，还含有可收缩的外毛细胞（OHC），它们与声音同步地主动收缩和伸长。然而，OHC在体内的运动仍然没有确定。本工作的目的是进行高分辨率和高精度的振动测量在活豚鼠与SC引入光谱域光学相干断层扫描系统（SD-OCT）。在这项研究中，为了揭示SC源在短的总采集时间内记录低反射材料中的有效贡献，我们比较了SC引入SD-OCT（SCSD-OCT）和传统SD-OCT的性能。作为无生命的比较对象，我们记录了镜子，压电致动器和玻璃窗。对于生物材料中的测量，我们使用体内/离体豚鼠耳蜗。我们的研究实现了SD-OCT系统的优化，用于在耳蜗感觉上皮（称为Corti器官）中进行高分辨率的体内振动测量。通过引入超连续谱（SC）光源和减少总采集时间，提高了轴向分辨率，克服了在生物噪声存在下记录低反射材料的困难。SC源的高功率使系统能够在反射镜上实现1.72 ± 0.00 μm的空间分辨率，并且减少总采集时间有助于亚纳米级振动测量的高空间精度。我们的研究结果揭示了振动的顶/基底区的OHC和细胞外基质，基底膜。
Sound evokes sub-nanoscale vibration within the sensory epithelium. The epithelium contains not only immotile cells but also contractile outer hair cells (OHCs) that actively shrink and elongate synchronously with the sound. However, the in vivo motion of OHCs has remained undetermined. The aim of this work is to perform high-resolution and -accuracy vibrometry in live guinea pigs with an SC-introduced spectral-domain optical coherence tomography system (SD-OCT). In this study, to reveal the effective contribution of SC source in the recording of the low reflective materials with the short total acquisition time, we compare the performances of the SC-introduced SD-OCT (SCSD-OCT) to that of the conventional SD-OCT. As inanimate comparison objects, we record a mirror, a piezo actuator, and glass windows. For the measurements in biological materials, we use in/ex vivo guinea pig cochleae. Our study achieved the optimization of a SD-OCT system for high-resolution in vivo vibrometry in the cochlear sensory epithelium, termed the organ of Corti, in mammalian cochlea. By introducing a supercontinuum (SC) light source and reducing the total acquisition time, we improve the axial resolution and overcome the difficulty in recording the low reflective material in the presence of biological noise. The high power of the SC source enables the system to achieve a spatial resolution of 1.72 ± 0.00 μm on a mirror and reducing the total acquisition time contributes to the high spatial accuracy of sub-nanoscale vibrometry. Our findings reveal the vibrations at the apical/basal region of OHCs and the extracellular matrix, basilar membrane.