Astroglial cells in vitro are heterogeneous with respect to expression of the α1‐adrenergic receptor
Astroglial cells in vitro are heterogeneous with respect to expression of the α1‐adrenergic receptor
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体外星形胶质细胞在 α1 肾上腺素能受体的表达方面存在异质性
作者：
L. Lerea;K. McCarthy
已使用多种方法来检查培养的星形胶质细胞的神经配体受体的表达。这些研究的结果清楚地表明，这些细胞表现出大多数（如果不是全部）已知与中枢神经元相关的不同受体。然而，确定是否存在多个可以根据神经配体受体的互补来区分的星形胶质细胞群体变得更加困难。为了解决这个问题，我们建立了一种放射性配体结合测定，使我们能够在免疫细胞化学定义的神经细胞上可视化α1-肾上腺素能受体（α1-AR）。饱和度、时间过程和竞争结合实验确定 125I-HEAT 可用于识别免疫细胞化学定义的星形胶质细胞上的 α1-AR 结合位点。我们的结果表明，大约 66% 的皮质多边形星形胶质细胞表达可检测数量的 α1-AR。 3H-胸苷标记实验表明分裂和非分裂星形胶质细胞均表现出α1-AR。这些结果与 β-肾上腺素能受体表达 (β-AR) 的研究形成鲜明对比，其中 100% 的多边形星形胶质细胞表达 β-AR。带有突起的星形胶质细胞也表现出α1-AR，这与这些细胞缺乏β-AR表达形成鲜明对比。这里提出的结果表明，星形胶质细胞与神经元一样，其表达的受体不同，因此可能能够选择性地响应细胞外刺激。
A wide variety of approaches have been used to examine the expression of neuroligand receptors by cultured astroglial cells. The results of such studies make it clear that these cells exhibit most, if not all, of the different receptors known to be associated with central neurons. However, it has been more difficult to determine if there are multiple populations of astroglia that can be distinguished on the basis of their complement of neuroligand receptors. To address this question, we established a radioligand binding assay that enabled us to visualize α1‐adrenergic receptors (α1‐ARs) on immunocytochemically defined neural cells. Saturation, time course, and competition binding experiments determined that 125I‐HEAT could be used to identify α1‐AR binding sites on immunocytochemically defined astroglial cells. Our results indicate that approximately 66% of cortical polygonal astroglia express detectable numbers of α1‐ARs. 3H‐thymidine labeling experiments indicate that both dividing and nondividing astroglia exhibit α1‐ARs. These results greatly contrast with studies on β‐adrenergic receptor expression (β‐AR), in which 100% of polygonal astroglia express β‐ARs. Process‐bearing astroglia also exhibit α1‐ARs, which is in marked contrast to the lack of β‐AR expression by these cells. The results presented here suggest that astroglia, like neurons, differ in the receptors they express and therefore may be able to selectively respond to extracellular stimulation.
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影响因子：
21.1
作者：
Harden,TK
通讯作者：
Harden,TK
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影响因子：
3.6
作者：
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通讯作者：
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影响因子：
3.6
作者：
Morrow,AL;Creese,I
通讯作者：
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DOI：
--
发表时间：
1987
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Jagadeesh,G;Deth,RC
通讯作者：
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DOI：
--
发表时间：
1987
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Johnson,RD;Iuvone,PM;Minneman,KP
通讯作者：
Minneman,KP