Molecular characterization and expression of two ovarian lipoprotein receptors in the rainbow trout, Oncorhynchus mykiss
Molecular characterization and expression of two ovarian lipoprotein receptors in the rainbow trout, Oncorhynchus mykiss
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DOI：
10.1095/biolreprod58.5.1146
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发表时间：
1998-05-01
影响因子：
3.6
通讯作者：
Tyler, CR
中科院分区：
文献类型：
--
作者：
Prat, F;Coward, K;Tyler, CR
从虹鳟（Oncorhynchus mykiss）卵巢中分离并测序了编码两个脂蛋白受体的部分cdna。其中一种cdna （rt-LPR）含有低密度脂蛋白受体超家族受体的5个结构域特征。第二个cDNA （rt-LP[OS]R）与rt-LPR相似，但缺少105个编码o链糖结构域的碱基对。推导出的rt-LPR和rt-LP[OS]R的氨基酸序列与其他物种的极低密度脂蛋白和卵黄蛋白原受体具有75% ~ 80%的一致性。rt-脂蛋白受体mrna的大小约为3.5千碱基。rt-LPR在卵巢和体细胞组织中均有表达，而rt-LP[OS]R是卵巢特异性的。脂蛋白受体的信使RNA在卵黄形成前（< 0.3 mm）和卵黄形成早期（高达1mm）卵泡中都有高水平表达。利用逆转录聚合酶链反应，rt-LPR和rt-LP[OS]R mRNA的表达也在较大的卵黄形成卵泡（直径达2.5 mm）中检测到，但在卵黄形成晚期的卵泡或排卵卵子中未检测到。rt-LPR mRNA的序列、卵巢特异性和卵巢表达模式表明，它编码介导卵黄蛋白原摄取到卵巢的受体。
Partial cDNAs encoding two lipoprotein receptors were isolated and sequenced from the ovary in the rainbow trout (rt), Oncorhynchus mykiss. One of the cDNAs (rt-LPR) contained the 5 domains characteristic of receptors belonging to the low-density lipoprotein receptor superfamily. The second cDNA (rt-LP[OS]R) was similar to rt-LPR but lacked 105 base pairs encoding the O-linked sugar domain. The deduced amino acid sequences of the rt-LPR and rt-LP[OS]R had between 75% and 80% identity with very-low-density lipoprotein and vitellogenin receptors of other species. The rt-lipoprotein receptor mRNAs were approximately 3.5 kilobases in size. The rt-LPR was expressed in both the ovary and somatic tissues, whereas the rt-LP[OS]R was ovary-specific. Messenger RNA for the lipoprotein receptor(s) was expressed at high levels in both pre-vitellogenic (< 0.3 mm) and early vitellogenic (up to 1 mm) follicles. Using reverse transcription-polymerase chain reaction, expression of rt-LPR and rt-LP[OS]R mRNA was also detected in larger vitellogenic follicles (up to 2.5 mm in diameter) but not in follicles in late vitellogenesis or in ovulated eggs. The sequence, ovary specificity, and pattern of ovarian expression of the rt-LPR mRNA suggest that it encodes the receptor that mediates vitellogenin uptake into the ovary.