New fimbrial gene cluster of S-fimbrial adhesin family
New fimbrial gene cluster of S-fimbrial adhesin family
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DOI：
10.1128/iai.68.10.5901-5907.2000
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发表时间：
2000-10-01
影响因子：
3.1
通讯作者：
Ron, EZ
中科院分区：
文献类型：
--
作者：
Babai, R;Stern, BE;Ron, EZ
菌毛粘附素介导宿主细胞粘附，由大多数致病性大肠杆菌分离株产生，这些毒力因子在细菌定植的初始阶段以及确定宿主和组织特异性中起重要作用。已知属于血清型O 78的分离株在家畜和人类中引起多种临床综合征，并已显示产生几种类型的粘附菌毛。我们研究了禽败血症大肠杆菌菌毛粘附素。大肠杆菌O 78血清型分离株的菌毛遗传结构分析; coli）基因簇表明其属于S-菌毛粘附素家族。7个开放阅读框编码的主要和次要结构亚基，其中大部分显示出高度的同源性，相应的Sfa和Foc决定簇。最不保守的开放阅读框架是facS，编码一种已知在确定其他S-菌毛基因簇中的粘附特异性中起重要作用的蛋白质。
Fimbrial adhesins that mediate attachment to host cells are produced by most virulent Escherichia coli isolates, These virulence factors play an important role in the initial stages of bacterial colonization and also in determination of the host and tissue specificity. Isolates belonging to serotype O78 are known to cause a large variety of clinical syndromes in farm animals and humans and have been shown to produce several types of adherence fimbriae. We studied the fimbrial adhesin from an avian septicemic E. coli isolate of serotype O78, Analysis of the genetic organization of the fac (fimbria of avian E. coli) gene cluster indicates that it belongs to the S-fimbrial adhesin family. Seven open reading frames coding for major and minor structural subunits were identified, and most of them showed a high degree of homology to the corresponding Sfa and Foc determinants. The least-conserved open reading frame was facS, encoding a protein known to play an important role in determining adherence specificity in other S-fimbrial gene clusters.