Phylogenetic analysis of Aspergillus species using DNA sequences from four loci
Phylogenetic analysis of Aspergillus species using DNA sequences from four loci
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DOI：
10.3852/mycologia.100.2.205
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发表时间：
2008-03-01
期刊：
影响因子：
2.8
通讯作者：
Peterson, Stephen W.
中科院分区：
文献类型：
--
作者：
Peterson, Stephen W.
测定了来自约100株大肠杆菌的β微管蛋白（BT 2）、钙调蛋白（CF）、ITS和lsu rDNA（ID）以及RNA聚合酶II（RPB 2）的DNA序列。460个曲霉菌分离株。RPB 2和rDNA序列相结合，并分析，以确定在属和科Trichocomaceae的关系。正青霉属物种形成统计学支持的分支，起源于曲霉属分支中。A.堆叠我们，A. malodoratus和H. paradoxus是正青霉属进化枝的成员。A. zonatus，黄毛菊A. clavatoflvus和W. spinulosa与Hamigera sp.一起沿着出现在一个分支中。除了这些例外的种，曲霉属的种和组出现在三个强烈支持的分支中，这些分支是从一个多分裂体下降的。作为一个单系群的组Versicolores仅包括A. versicolor和A. Sydowii和是多余的。其他部分保留，但进行了修改。所有四个位点都用于种间界线的系谱一致性分析。Fennellia flavipes和F. nivea与它们假定的无性型A不是同种的。flavipes和A.妮维雅在系谱一致性分析中，发现了一些种的同义词和20多个未描述的分类群。新发现的分类群将在其他地方描述。可能的旁系同源基因片段用BT2引物在Nidulaeus、Usti和Nigri切片中扩增。在系谱一致性分析中使用非同源序列可能导致错误的结论，因此在这些部分的分析中不使用BT2序列。
DNA sequences were determined for beta tubulin (BT2), calmodulin (CF), ITS and lsu rDNA (ID) and RNA polymerase II (RPB2) from ca. 460 Aspergillus isolates. RPB2 and rDNA sequences were combined and analyzed to determine relationships in the genus and in the family Trichocomaceae. Eupenicillium species form a statistically supported clade with origins among the Aspergillus clades. A. crystallinus, A. malodoratus and H. paradoxus are members of the Eupenicillium clade. A. zonatus, A. clavatoflvus and W. spinulosa occur in a clade along with Hamigera sp. Other than these exceptional species, Aspergillus species and sections occur on three strongly supported clades that descend from a polytomy. Section Versicolores as a monophyletic group includes only A. versicolor and A. sydowii and is superfluous. The other sections were retained but modified. All four loci were used in genealogical concordance analysis of species boundaries. Fennellia flavipes and F. nivea are not conspecific with their supposed anamorphs A. flavipes and A. nivea. Synonymies were found for some species and more than 20 undescribed taxa were identified in genealogical concordance analysis. Newly discovered taxa will be described elsewhere. Possibly paralogous gene fragments were amplified with the BT2 primers in sections Nidulantes, Usti and Nigri. Use of nonhomologous sequences in genealogical concordance analysis could lead to false conclusions and so BT2 sequences were not used in analysis of those sections.