Age-associated differences in prevalence of group A streptococcal type-specific M antibodies in children
Age-associated differences in prevalence of group A streptococcal type-specific M antibodies in children
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DOI：
10.1007/s00431-008-0819-6
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发表时间：
2009-06-01
影响因子：
3.6
通讯作者：
Shulman, Stanford T.
中科院分区：
文献类型：
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作者：
Jaggi, Preeti;Dale, James B.;Shulman, Stanford T.
我们先前对A组链球菌（GAS）咽炎的分子流行病学研究表明，在北美与小儿咽炎相关的最常见的emm类型为12、1、28和4。我们以前曾报道过，在整个儿童时期，由于12型和4型emm引起的小儿咽炎的比例随着年龄的增长而下降。我们假设这是由于儿童时期常见GAS M蛋白氨基末端类型特异性区域抗体的年龄相关性获得。我们试图通过使用M12、1、28和4的ELISA在健康儿童的血清中证明这一点。酶联免疫吸附试验（ELISA）使用化学合成的肽复制的氨基末端类型的特定区域的M蛋白进行血清从四个年龄组的健康儿童（组I：3-6岁，组II：7-10岁，组III：11-14岁，组IV：15-18岁）。ELISA数据与血清和M1 GAS亚组的调理吞噬试验相关。来自健康12-20月龄儿童的血清用作阴性对照。我们的研究结果表明，阳性率最高的是M12，这也表明在年龄较大的儿童进行性血清阳性。对于其他血清型，11-14岁年龄组的血清阳性率最高。针对M1的ELISA抗体的存在与调理吞噬活性相关，调理吞噬活性是先前研究的免疫保护指标。
Our prior studies of the molecular epidemiology of group A streptococcus (GAS) pharyngitis indicated that the most common emm types associated with pediatric pharyngitis in North America were 12, 1, 28, and 4. We previously reported that the proportions of pediatric pharyngitis due to emm types 12 and 4 decreased with increasing age throughout childhood. We hypothesized that this is due to age-associated acquisition of antibodies to the amino-terminal type-specific region of common GAS M proteins during childhood. We sought to demonstrate this in sera from healthy children by using ELISAs for M 12, 1, 28, and 4. Enzyme-linked immunosorbent assays (ELISA) using chemically synthesized peptides copying amino-terminal type-specific regions of the M proteins were performed on sera from four age groups of healthy children (group I: 3-6 years, group II: 7-10 years, group III: 11-14 years, group IV: 15-18 years). ELISA data were correlated with opsonophagocytic assays for a subset of sera and M 1 GAS. Sera from healthy 12-20-month-old children were used as negative controls. Our results showed that the highest percentage of positivity was for M12, which also showed progressive seropositivity in older children. For the other serotypes, the highest seroprevalence rates were in the 11-14-year-old age group. The presence of ELISA antibodies against M1 correlated with opsonophagocytic activity, a previously studied indicator of immunologic protection.