Synergetic effect of SLN-curcumin and LDH-5-Fu on SMMC-7721 liver cancer cell line.
Synergetic effect of SLN-curcumin and LDH-5-Fu on SMMC-7721 liver cancer cell line.
复制标题
DOI：
10.1089/cbr.2012.1445
复制
发表时间：
2013-09
影响因子：
3.4
通讯作者：
Rongrong Zhu;Xianzheng Wu;Yu Xiao;Bo Gao;Q. Xie;Hui Liu;Shilong Wang
中科院分区：
文献类型：
--
作者：
Rongrong Zhu;Xianzheng Wu;Yu Xiao;Bo Gao;Q. Xie;Hui Liu;Shilong Wang
据报道，姜黄素和5-氟尿嘧啶（5-Fu）具有抗癌潜力，并对某些癌细胞系表现出一定的协同作用。然而，生物利用度差和代谢快限制了它们的医学应用。在本研究中，我们分别用固体脂质纳米颗粒（SLN）、5-Fu和层状双氢氧化物（LDH）封装姜黄素，并测试其特性和抗癌潜力。经透射电镜检测，SLN-姜黄素和LDH-5-Fu分别为100和60 nm，负载效率分别为28%±2.5%和16.7%±1.8%。此外，SLN-姜黄素和LDH-5-Fu对SMMC-7721细胞表现出显着的协同作用，其协同作用强于普通药物，其中载药纳米载体的Idrug仅为0.315。 FACS分析显示，SLN-姜黄素和LDH-5-Fu联合诱导SMMC-7721细胞凋亡80.1%，是两种普通载药载体总和的1.7倍。结果证明了纳米胶囊姜黄素和5-Fu具有显着的协同抗癌潜力，可以进一步探索其用于其他癌症的治疗。
Curcumin and 5-Fluorouracil (5-Fu) have been reported to have anticancer potentials and show certain synergetic effect on some cancer cell lines. However, the poor bioavailability and rapid metabolism limited their medical application. In this study, we encapsulated curcumin with solid lipid nanoparticles (SLN), 5-Fu with Layered double hydroxides (LDHs) separately and tested its properties and anticancer potentials. SLN-curcumin and LDH-5-Fu were determined to be 100 and 60 nm by Transmission Electron Microscopy detection, and the loading efficiency were 28%±2.5% and 16.7%±1.8%, individually. Furthermore, SLN-curcumin and LDH-5-Fu showed a significantly synergetic effect on SMMC-7721 cell stronger than plain drugs together, of which the Idrug loaded nano-carriers was only 0.315. FACS analysis revealed that the combination of SLN-curcumin and LDH-5-Fu induced 80.1% apoptosis in SMMC-7721 cells, which were 1.7-folds of the sum of the two plain drug loaded carriers. The results demonstrated the significant synergetic anticancer potentials of nano-encapsulated curcumin and 5-Fu, which could be further explored for the treatment of other carcinoma.