Identification of Staphylococcus aureus brain abscesses:: Rat and human studies with 1H MR spectroscopy
Identification of Staphylococcus aureus brain abscesses:: Rat and human studies with 1H MR spectroscopy
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DOI：
10.1148/radiol.2361040869
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发表时间：
2005-07-01
期刊：
影响因子：
19.7
通讯作者：
Mountford, CE
中科院分区：
文献类型：
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作者：
Himmelreich, U;Accurso, R;Mountford, CE
目的：确定统计分类策略（SCS）的可行性，以及细菌和宿主来源代谢物的身份，这些代谢物可能导致来自对照、胶质瘤和葡萄球菌脓肿的活检材料的金黄色葡萄球菌脓肿的磁共振（MR）光谱中最具区别性的区域。材料和方法：人类和动物研究获得了伦理委员会的批准，并获得了患者的知情同意。建立大鼠S型脑脓肿模型。在启动之后。用体外磁共振光谱分析了脓液（n = 62）和对照组（n = 37）的代谢物谱，并与大鼠胶质瘤（n = 27）的数据进行了比较。SCS、最优区域选择和两两分类器的发展使脓肿（n = 42，第6-8天）的MR谱与多形性胶质母细胞瘤和对照组区分开来。将动物脓肿脓液的磁共振光谱与葡萄球菌性脑脓肿患者（7例，年龄6 ~ 67岁）的体内磁共振光谱和金黄色葡萄球菌性脑脓肿患者的体外磁共振光谱进行比较。结果：47只大鼠中有42只（89%）在立体定向注射后6-8天出现组织学证实的脓肿。动物模型中脓肿与多形性胶质母细胞瘤的MR谱相似。在大鼠或人的金黄色葡萄球菌脓肿中未检测到由厌氧菌引起的脓肿的典型代谢物（醋酸盐、琥珀酸盐、氨基酸）。对照、脓肿和胶质瘤的磁共振光谱结果通过SCS区分，准确率为99%。二维相关光谱分析表明，谷氨酰胺和/或谷氨酸和天冬氨酸可能有助于成功分类。结论：用非培养方法在动物模型脓肿中检测到金黄色葡萄球菌。(c) RSNA， 2005年。
PURPOSE: To determine the feasibility of a statistical classification strategy (SCS) and the identity of metabolites of bacterial and host origins that potentially contributed to the most discriminatory regions of magnetic resonance (MR) spectra from Staphylococcus aureus abscesses of biopsy material from controls, gliomas, and staphylococcal abscesses.MATERIALS AND METHODS: Human and animal study received ethics committee approval, and informed patient consent was obtained. A rat model of S oureus brain abscess was developed. after initiation. Metabolite profiles in pus (n = 62) and controls (n = 37) were characterized with ex vivo MR spectroscopy and compared with data from rat gliomas (n = 27). SCS, optimal region selection, and development of pairwise classifiers allowed MR spectra of abscesses (n = 42, day 6-8) to be distinguished from those of glioblastoma multiforme and controls. MR spectroscopy profiles of pus from animal abscesses were compared with in vivo MR spectra from patients with staphylococcal brain abscesses (n = 7, aged 6-67 years) and ex vivo pus MR spectra from patients with S aureus abscesses.RESULTS: Histologically confirmed abscesses were present 6-8 days after stereotactic injection of S oureus in 42 of 47 rats (89%). MR spectra of abscesses and glioblastoma multiforme in the animal model were similar. Typical metabolites of abscesses due to anaerobe bacteria (acetate, succinate, amino acids) were not detectable in S aureus abscesses in rats or humans. MR spectroscopic findings from controls, abscesses, and gliomas were distinguished by means of SCS with an accuracy of 99%. Analysis of the most discriminatory regions with two-dimensional correlation spectra indicated that glutamine and/or glutamate and aspartate potentially contributed to successful classification.CONCLUSION: S aureus is detectable in abscesses with a non-culture-based method in an animal model. ((c)) RSNA, 2005.