A hybrid plasmid for expression of toxic malarial proteins in Escherichia coli
A hybrid plasmid for expression of toxic malarial proteins in Escherichia coli
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DOI：
10.1016/s0166-6851(01)00354-1
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发表时间：
2001-10-01
影响因子：
1.5
通讯作者：
Menz, RI
中科院分区：
文献类型：
--
作者：
Cinquin, O;Christopherson, RI;Menz, RI
进行疟疾酶乳清酸核苷-5 ′单磷酸脱羧酶（ODCase，EC 4.1.1.23）的过表达。用疟疾ODCase表达载体pET-ODC转化E.杆菌E.与其它疟原虫表达质粒的细胞比较，表明疟原虫ODCase对E.大肠杆菌在用异丙基-β-D-硫代半乳糖苷诱导之前以低水平产生。结论是新的杂交质粒，pMICO，促进疟疾ODCase的过表达，并可能是有用的其他基因，利用rareE的过表达。大肠杆菌和其蛋白质产品是有毒的E。杆菌
Overexpression of the malarial enzyme, orotidine-5′monophosphate decarboxylase (ODCase, EC 4.1.1.23) was performed. The malarial ODCase expression vector (pET-ODC) was used to transformE. coli. The slower growth ofE. colicontaining pET-ODC compared with cells containing other malarial expression plasmids, suggested that the malarial ODCase was toxic toE. coliand produced at low levels prior to induction with isopropyl-beta-D-thiogalactoside. It is concluded that the new hybrid plasmid, pMICO, facilitates the overexpression of malarial ODCase and is likely to be useful for the over-expression of other genes which utilize rareE. colicodons and whose protein products are toxic toE. coli.