PHILADELPHIA CHROMOSOMAL BREAKPOINTS ARE CLUSTERED WITHIN A LIMITED REGION, BCR, ON CHROMOSOME-22
PHILADELPHIA CHROMOSOMAL BREAKPOINTS ARE CLUSTERED WITHIN A LIMITED REGION, BCR, ON CHROMOSOME-22
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DOI：
10.1016/0092-8674(84)90077-1
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发表时间：
1984-01-01
期刊：
影响因子：
64.5
通讯作者：
GROSVELD, G
中科院分区：
文献类型：
--
作者：
GROFFEN, J;STEPHENSON, JR;GROSVELD, G
从1例慢性粒细胞白血病（CML）患者的22号染色体上鉴定出46 kb的人DNA，并使用费城（Ph“）易位断裂点结构域特异性探针进行分子克隆。用从该克隆区域分离的探针检测了19例CML患者22号染色体上的DNA重排。在17例患者中，染色体断裂点被发现在一个有限的区域内高达5.8 kb，为术语断点簇区（bcr）的建议。2例bcr内无重排的患者缺乏Ph“染色体。Ph阳性CML患者bcr内染色体断裂点的高度特异性存在强烈提示bcr参与了这类白血病。
Forty-six kb [kilabases] of human DNA from chromosome 22 were identified and molecularly cloned using a probe specific for the Philadelphia (Ph'') translocation breakpoint domain of 1 chronic myelocytic leukemia (CML) patient. DNA of 19 CML patients were examined for rearrangements on chromosome 22 with probes isolated from this cloned region. In 17 patients, chromosomal breakpoints were found within a limited region of up to 5.8 kb, for which the term breakpoint cluster region (bcr) is proposed. The 2 patients having no rearrangements within bcr lacked the Ph'' chromosome. The highly specific presence of a chromosomal breakpoint within bcr in Ph''-positive CML patients strongly suggests the involvement of bcr in this type of leukemia.