Arabidopsis trehalose-6-phosphate synthase 1 is essential for normal vegetative growth and transition to flowering
Arabidopsis trehalose-6-phosphate synthase 1 is essential for normal vegetative growth and transition to flowering
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DOI：
10.1104/pp.104.039743
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发表时间：
2004-06-01
期刊：
影响因子：
7.4
通讯作者：
Smeekens, SCM
中科院分区：
文献类型：
--
作者：
van Dijken, AJH;Schluepmann, H;Smeekens, SCM
在复活植物和酵母中，海藻糖具有保护胁迫的功能，但在其他植物中缺乏可测量的海藻糖就排除了这一功能。被子植物中海藻糖生物合成途径的鉴定提出了关于非复活植物中海藻糖代谢功能的问题。我们先前在拟南芥海藻糖生物合成基因AtTPS1中发现了一个突变体。Tps1突变纯合的植物不会发育成熟种子(Eastmond等人，2002年)。AtTPS1的表达分析及其启动子的空间和时间活性表明，该基因在发育的种子灌浆阶段之外也是活跃的。在所有被分析的器官中都观察到了低水平的表达，在新陈代谢库中达到顶峰，如花蕾、成熟的角果和年轻的莲座叶。被逮捕的tps 1/tps 1。使用地塞米松诱导的AtTPS1表达系统可以挽救胚胎状态，从而能够产生纯合子突变植株。当AtTPS1表达缺失时，这种突变植物表现出根生长减少，这与根分生组织区域减少相关。此外，tps1/tps1植物生长迟缓，并在其一生中保持生殖能力。海藻糖-6-磷酸合成酶1在拟南芥中的缺失阻碍了向开花的过渡。
In resurrection plants and yeast, trehalose has a function in stress protection, but the absence of measurable amounts of trehalose in other plants precludes such a function. The identification of a trehalose biosynthetic pathway in angiosperms raises questions on the function of trehalose metabolism in nonresurrection plants. We previously identified a mutant in the Arabidopsis trehalose biosynthesis gene AtTPS1. Plants homozygous for the tps1 mutation do not develop mature seeds (Eastmond et al., 2002). AtTPS1 expression analysis and the spatial and temporal activity of its promoter suggest that this gene is active outside the seed-filling stage of development as well. A generally low expression is observed in all organs analyzed, peaking in metabolic sinks such as flower buds, ripening siliques, and young rosette leaves. The arrested tps1/tps1. embryonic state could be rescued using a dexamethasone-inducible AtTPS1 expression system enabling generation of homozygous mutant plants. When depleted in AtTPS1 expression, such mutant plants show reduced root growth, which is correlated with a reduced root meristematic region. Moreover, tps1/tps1 plants are retarded in growth and remain generative during their lifetime. Absence of Trehalose-6-Phosphate Synthase 1 in Arabidopsis plants precludes transition to flowering.