Detection of early metabolic alterations in the ocular fundus of diabetic patients by time-resolved autofluorescence of endogenous fluorophores
Detection of early metabolic alterations in the ocular fundus of diabetic patients by time-resolved autofluorescence of endogenous fluorophores
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通过内源性荧光团的时间分辨自发荧光检测糖尿病患者眼底的早期代谢变化
DOI：
10.1117/12.889109
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发表时间：
2011
影响因子：
3.1
通讯作者：
U. A. Mueller
中科院分区：
文献类型：
--
作者：
Dietrich Schweitzer;M. Klemm;S. Quick;Lydia Deutsch;Susanne Jentsch;Martin Hammer;Jens Dawczynski;C. H. Kloos;U. A. Mueller
在糖尿病患者的人眼底测量时间分辨自体荧光（FLIM）允许在糖尿病视网膜病变的迹象可见之前检测早期病理改变。通过Jena荧光寿命激光扫描检眼镜在两个光谱通道（K1：490-560 nm，K2：560- 700 ps）中应用时间相关单光子计数（TCSPC）进行测量。荧光由448 nm处的70 ps脉冲（FWHM）激发。荧光强度的衰减是三指数近似的。在健康受试者和糖尿病患者中，在相同大小和位置的字段中比较振幅，寿命和相对贡献的频率。最敏感的参数是在短波长通道中的寿命T2，其对应于神经元视网膜。寿命的变化表明在糖尿病视网膜病变前期游离NADH的损失和蛋白结合的NADH的贡献增加。
Measurements of time-resolved autofluorescence (FLIM) at the human ocular fundus of diabetic patients permit the detection of early pathologic alterations before signs of diabetic retinopathy are visible. The measurements were performed by the Jena Fluorescence Lifetime Laser Scanner Ophthalmoscope applying time-correlated single photon counting (TCSPC) in two spectral channels (K1: 490-560 nm, K2:560-700ps). The fluorescence was excited by 70 ps pulses (FWHM) at 448 nm. The decay of fluorescence intensity was triple-exponentially approximated. The frequency of amplitudes, lifetimes, and relative contributions was compared in fields of the same size and position in healthy subjects and in diabetic patients. The most sensitive parameter was the lifetime T2 in the short-wavelength channel, which corresponds to the neuronal retina. The changes in lifetime point to a loss of free NADH and an increased contribution of protein-bound NADH in the pre-stage of diabetic retinopathy.