Drosophila clipper/CPSF 30K is a post-transcriptionally regulated nuclear protein that binds RNA containing GC clusters
Drosophila clipper/CPSF 30K is a post-transcriptionally regulated nuclear protein that binds RNA containing GC clusters
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DOI：
10.1093/nar/26.7.1597
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发表时间：
1998-04-01
影响因子：
14.9
通讯作者：
Tolias, PP
中科院分区：
文献类型：
--
作者：
Bai, CY;Tolias, PP
哺乳动物前信使核糖核酸的重要组成部分。3‘端加工机制是一种多聚体蛋白质复合体，被称为切割和多腺苷化特异性因子(CPSF)，是果蝇的黑腹果蝇基因CLP(CLP)，编码CPSF 30K亚基的同源基因，我们先前已经证明CLP具有N端的内源性核溶解活性，其mRNA的相对表达在果蝇发育过程中是波动的。在本研究中，我们报告了CLP的C末端，包含两个CCHC锌指关节，赋予了对包含G-和/或富C簇的RNA的结合偏好。我们还首次证明了高度保守的CPSF 30K家族的一个成员是一个核和发育调节蛋白。尽管在整个胚胎发育过程中都可以检测到CLP转录本，但CLP蛋白并不存在。我们证明了胚胎中CLP mRNA的转录后调控是通过一个不涉及Poly(A)尾长缩短的过程进行的。因此，前mRNA 3‘端加工机械的一个关键组成部分在发育过程中受到转录后调控。这些结果支持存在一种独特的机制，通过调控细胞核中的前-mRNAs来控制真核基因的表达。
An essential component of the mammalian pre-mRNA. 3'-end processing machinery isa multimeric protein complex known as cleavage and polyadenylation specificity factor (CPSF), The Drosophila melanogaster gene, clipper (clp), encodes a homolog of the CPSF 30K subunit, We have shown previously that CLP possesses N-terminal endoribonucleolytic activity and that the relative expression of its mRNA fluctuates during fly development. In the present study, we report that CLP's C-terminus, containing two CCHC zinc knuckles, confers a binding preference for RNAs that contain G- and/or C-rich clusters. We also show, for the first time, that a member of the highly conserved CPSF 30K family is a nuclear and developmentally regulated protein. Though clp transcripts are detectable throughout embryogenesis, CLP protein is not present. We demonstrate that post-transcriptional regulation of clp mRNA in the embryo occurs by a process that does not involve poly(A) tail length shortening. Thus, a key component of the pre-mRNA 3'-end processing machinery is subject to post-transcriptional regulation during development. These results support the existence of a distinct mechanism controlling eukaryotic gene expression through the regulated processing of pre-mRNAs in the nucleus.