Metabolomic profiling reveals distinct patterns of tricarboxylic acid disorders in blood stasis syndrome associated with coronary heart disease
Metabolomic profiling reveals distinct patterns of tricarboxylic acid disorders in blood stasis syndrome associated with coronary heart disease
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代谢组学分析揭示了与冠心病相关的血瘀证中三羧酸紊乱的独特模式
DOI：
10.1007/s11655-015-2401-1
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发表时间：
2016
影响因子：
2.9
通讯作者：
Wang Wei
中科院分区：
文献类型：
--
作者：
Wang Yong;Li Chun;Chang Hong;Lu Ling hui;Qiu Qi;Ouyang Yu lin;Yu Jun da;Guo Shu zhen;Han Jing;Wang Wei
目的探讨冠心病血瘀证的代谢组学特征。将15头小型猪按随机数字表法分为模型组（n=9）和对照组（n=6）。治疗4周后，采用全自动血液流变学分析仪检测血浆血液流变学，指标包括红细胞压积、血浆粘度、全血粘度、刚性指数和血沉;采用超声心动图仪检测左室收缩末内径（LVED）、左室舒张末内径（LVEDd）、射血分数（EF）、短轴缩短率（FS）等指标评价心功能。结果血液流变学检测结果显示，模型组与对照组相比，全血低切粘度降低，血浆粘度升高，血液流变学检测结果显示，模型组与对照组相比，全血低切粘度降低，血浆粘度升高。全血高切还原粘度和全血低切还原粘度显著升高（P<0.05）。超声心动图结果显示，心脏EF和FS存在显著差异（P<0.05），EF值下降至50%或以下。GC-MS数据显示，主成分分析可以清楚地将BSS动物与对照组动物区分开来。丰富的京都基因百科全书和基因组生物学途径的结果表明，所涉及的模式与能量代谢功能障碍，包括糖和脂质障碍，特别是在糖酵解/糖异生，半乳糖代谢和腺苷三磷酸结合盒transporters.ConclusionsGlucose代谢和脂质代谢障碍的主要贡献者冠心病BSS的证型分类。
ObjectiveTo investigate the underlying metabolomic profifiling of coronary heart disease (CHD) with blood stasis syndrome (BSS).MethodsCHD model was induced by a nameroid constrictor in Chinese miniature swine. Fifteen miniature swine were randomly divided into a model group (n=9) and a control group (n=6), respectively according to arandom number table. After 4 weeks, plasma hemorheology was detected by automatic hemorheological analyzer, indices including hematocrit, plasma viscosity, blood viscosity, rigidity index and erythrocyte sedimentation rate; cardiac function was assessed by echocardiograph to detect left ventricular end-systolic diameter (LVED), left ventricular end-diastolic diameter (LVEDd), ejection fraction (EF), fractional shortening (FS) and other indicators. Gas chromatography coupled with mass spectrometry (GC-MS) and bioinformatics were applied to analyze spectra of CHD plasma with BSS.ResultsThe results of hemorheology analysis showed signifificant changes in viscosity, with low shear whole blood viscosity being lower and plasma viscosity higher in the model group compared with the control group. Moreover, whole blood reduction viscosity at high shear rate and whole blood reduction viscosity at low shear rate increased signifificantly (P<0.05). The echocardiograph results demonstrated that cardiac EF and FS showed signifificant difference (P<0.05), with EF values being decreased to 50% or less. The GC-MS data showed that principal component analysis can clearly separate the animals with BSS from those in the control group. The enriched Kyoto Encyclopedia of Genes and Genomes biological pathways results suggested that the patterns involved were associated with dysfunction of energy metabolism including glucose and lipid disorders, especially in glycolysis/gluconeogenesis, galactose metabolism and adenosine-triphosphate-binding cassette transporters.ConclusionsGlucose metabolism and lipid metabolism disorders were the major contributors to the syndrome classifification of CHD with BSS.